常温常压下等离子体灭活病毒效果的研究

目的观察常温常压下等离子体对流感病毒和脊髓灰质炎病毒的灭活效果。方法采用细胞培养法和基因扩增法,对常温常压下等离子体灭活悬液内病毒的效果进行了检测。结果该等离子体发生器置于本试验柜内启动运行4 h,对悬液内H3N2流感病毒灭活对数值为2.71;对悬液内脊髓灰质炎病毒Ⅰ型疫苗株灭活对数值为1.2。流感病毒和脊髓灰质炎病毒经等离子体作用至最长时间为4 h后,其核酸浓度均无明显变化。结论常温常压下等离子体对流感病毒和脊髓灰质炎病毒具不同程度的灭活效果,但其对两种病毒的核酸无破坏作用。

STUDY ON VIRUS INACTIVATION EFFICACY OF NON -THERMAL ATMOSPHERIC -PRESSURE PLASMA DISINFECTOR

Objective To observe the inactivation efficiency on influenza and poliomyelitis virus by non-thermal atmospheric-pressure plasma disinfector.Methods Cell cultural and real-time PCR techniques were used to detect the inactivation efficiency of non-thermal atmospheric-pressure plasma disinfector.Results The titer of influenza virus H3N2 decreased of 2.71 in plasma boxes with running for 4 hours.Poliomyelitis virus type I titer dropped about 1.20 in similar conditions.The nucleic acid load of both viruses did not change in plasma boxes for 4 hours.Conclusion The plasma device can inactivate influenza and poliomyelitis viruses,but can not damage the virus nucleic acid.