| 三七皂甙单体Rg1对失血性休克大鼠肠上皮细胞线粒体功能影响的研究 |
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| 引用本文： | 李伟文,方传勤,陆松敏,刘建仓,郭素清,程凤,王正国.三七皂甙单体Rg1对失血性休克大鼠肠上皮细胞线粒体功能影响的研究[J].创伤外科杂志,2010,12(3):255-258. |
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| 作者姓名： | 李伟文 方传勤 陆松敏 刘建仓 郭素清 程凤 王正国 |
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| 作者单位： | 1. 解放军第一医院
2. 第三军医大学野战外科研究所二室,创伤\烧伤与复合伤国家重点实验室,重庆,400042 |
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| 基金项目： | 国家"973"战创伤基础研究202课题 |
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| 摘 要： | 目的观察三七皂甙单体Rg1对失血性休克大鼠肠上皮细胞线粒体细胞色素氧化酶(COX)活性及膜电位的影响。方法健康Wistar大鼠24只,随机等分为4组:正常对照组(N)、失血性休克5小时组(HS)、单纯复苏对照组(RE)、复苏加Rg1治疗组(Rg),每组6只。采用荧光分光光度法检测线粒体膜电位;采用紫外分光光度法检测COX活性。结果失血性休克5小时大鼠肠上皮细胞线粒体摄取Rhoda-mine123减少,即膜电位水平显著降低(P0.01),单纯复苏组膜电位有轻微提高,复苏加Rg1治疗组线粒体膜电位明显提高,恢复到正常组水平。失血性休克5小时大鼠肠上皮细胞线粒体COX活性显著降低,单纯复苏后其活性仍然较低。复苏加Rg1治疗组COX活性较休克组比较有明显升高,并显著高于单纯复苏组。结论复苏加Rg1可显著提高失血性休克大鼠肠上皮细胞线粒体COX活性及线粒体膜电位。Rg1提高COX活性可能和提高该酶的产量和质量有关。
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| 关 键 词： | 失血性休克 肠上皮细胞 三七皂甙单体Rg1 细胞色素氧化酶 线粒体膜电位 |
Effect of panax notoginseng saponins Rg1 on mitochondrial function of small intestinal epithelial cells in hemorrhagic shock rats |
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| LI Wei-wen,FANG Chuan-qin,LU Song-ming,LIU Jian-cang,GUO Su-qing,CHENG Feng,WANG Zheng-guo.Effect of panax notoginseng saponins Rg1 on mitochondrial function of small intestinal epithelial cells in hemorrhagic shock rats[J].Journal of Traumatic Surgery,2010,12(3):255-258. |
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| Authors: | LI Wei-wen FANG Chuan-qin LU Song-ming LIU Jian-cang GUO Su-qing CHENG Feng WANG Zheng-guo |
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| Institution: | LI Wei-wen,FANG Chuan-qin,LU Song-ming,LIU Jian-cang,GUO Su-qing,CHENG Feng,WANG Zheng-guo(State Key Laboratory of Trauma,Burns , Combined Injury,Department 4,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Objective To study the effects of Rg1 isolated from panax notoginseng saponins(PNS)on mitochondrial cytochrome oxidase(COX)and membrane potential of small intestinal epithelial cells in hemorrhagic shock rats.Methods Twenty-four healthy Wistar rats were divided into 4 groups:control group(n=6),5 hour hemorrhagic shock group(n=6),simple resuscitation group(n=6),and resuscitation plus Rg1 treatment group(n=6).COX activity was determined by ultraviolet spectrophotometer,and mitochondrial membrane potential(MMP... |
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| Keywords: | hemorrhagic shock intestinal epithelial cells panax notoginseng saponins Rg1 cytochrome oxidase mitochondrial membrane potential |
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