重症胰腺炎性腹水对腹腔巨噬细胞功能影响

目的 观察大鼠重症胰腺炎性腹水对腹腔巨噬细胞功能及活性的影响，以了解腹腔巨噬细胞在重症胰腺炎病理生理演变和继发性胰腺感染中的作用，为重症胰腺炎的临床诊治提供指导。方法 通过离体细胞培养，分别在重症胰腺炎性腹水处理腹腔巨噬细胞1、3、6、12、14、24h后测定其吞噬功能、细胞活性扣肿瘤坏死因子分泌功能。结果 中性红吞噬功能试验、肿瘤坏死因子酶联免疫测定和噻唑蓝比色法细胞活性测定显示，随处理时间的延长，腹腔巨噬细胞的吞噬功能、肿瘤坏死因子分泌功能和细胞活性逐渐下降。结论 重症急性胰腺炎腹水降低了腹腔巨噬细胞的吞噬功能、细胞活性和肿瘤坏死因子分泌，是继发胰腺和胰周感染以及肠道细菌移位的促进因素之一。

The Influence of Ascites in Severe Acute Pancreatitis (SAP) on the Viability and Function of Peritoneal Macrophages

Objective To observe the influence of ascites in severe acute pancreatitis (SAP) on the function and viability of peritoneal macrophages in order to investigate the role of peritoneal macrophages in pathophysiological alteration and secondary pancreatic infection in SAP. Methods After the ascites of SAP models treated peritoneal macrophages for 1,3,6,12 and 24 hours in vitro, neutral red phagocytosis, cell viability and TNF secretion of peritoneal macrophages were determined respectively. Results The phagocytosis, cell viability and TNF secretion of macrophage all decreased with the treating time prolonged in the tests. Conclusions The ascites of SAP decreased the phagocytosis, viability and TNF secretion of peritoneal macrophages, and was one of the factors to promote secondary pancreatic and peripancreatic infection as well as bacterial translocation of gut.