miR-93-5p和miR-27a-3p在直肠癌组织中的表达及其意义

目的 探讨在直肠癌组织中呈现出特异性表达的miRNA与临床病理分期、肿瘤浸润深度、淋巴结转移等参数之间的关系及其可能的意义。方法 用微阵列基因芯片技术分析未经任何术前放化疗的71例直肠癌患者癌组织与癌旁组织间miRNA表达的区别,筛选出上调的miR-93-5p和下调的miR-27a-3p,扩大样本量后进行qRT-PCR验证,随后进行多种临床病理参数分析并探讨其可能存在的意义。结果 miR-27a-3p的表达在芯片检测中呈现出低表达,但在PCR验证时呈现出了高表达,且数据较为离散;miR-93-5p在两种检测方法中均显示出高表达的特性(癌组织表达量是癌旁组织的3.165倍,P=0.006),并与肿瘤体积(P=0.004)、治疗前癌胚抗原水平(P=0.001)及淋巴结转移数目(r=0.534,P=0.005)具有相关性,其中与治疗前癌胚抗原水平及淋巴结转移阳性组受侵数目存在正相关。结论 在直肠癌组织中存在特异性表达的miRNA。根据miR-93-5p和miR-27a-3p在直肠癌组织中的表达特点,miR-93-5p有望作为新型生物标记物,为直肠癌的临床诊疗提供参考价值。

Expression of miR-93-5p and miR-27a-3p in rectal cancer tissues and its clinical significance

Objective To investigate the miRNA expression profiles in rectal cancer tissues and their associations with clinical pathological stage, depth of tumor invasion, and lymph node metastasis, and to evaluate the potential of miRNA as diagnostic and prognostic markers of rectal cancer. Methods Human miRNA microarray was used to profile miRNA expression in rectal cancer tissues and matched adjacent normal tissues (n=71). The up-regulated miR-93-5p and down-regulated miR-27a-3p were screened out, and the top differentially expressed miRNA were validated by quantitative real-time polymerase chain reaction ( qRT-PCR) . The relationship between the expression of miRNA and clinical parameters was analyzed by ANOVA and Spearman correlation. Results The expression of miR-27a-3p was down-regulated in miRNA microarray, but was up-regulated in qRT-PCR analysis;the data were relatively discrete. The expression of miR-93-5p was up-regulated in both miRNA microarray and qRT-PCR analysis;the expression level of miR-93-5p in rectal cancer tissues was 3165 times that in adjacent normal tissues ( P=00058);the expression level was correlated with tumor volume ( P= 0004 ) , and was positively correlated with the level of carcinoembryonic antigen ( CEA) before treatment ( P=0001) and the number of lymph nodes metastases (rs=0534, P=0005). Conclusions There is a differential miRNA expression pattern between rectal cancer tissues and matched adjacent normal tissues. The miR-93-5p is highly up-regulated in rectal cancer tissues and may serve as a diagnostic and prognostic marker of rectal cancer.