骨髓间充质干细胞移植治疗犬急性放射性肠病临床观察

目的建立比格犬急性放射性肠病的损伤模型,观察骨髓间充质干细胞移植治疗犬急性放射性肠病的临床效果。方法将35只比格犬随机分为空白对照组(A组)、10 Gy单独照射组(B组)、12 Gy单独照射组(C组)、14 Gy单独照射组(D组)、10 Gy照射后干细胞治疗组(E组)、12 Gy照射后干细胞治疗组(F组)及14 Gy照射后干细胞治疗组(G组),每组5只。对比格犬腹部进行X线调强照射,观察单独照射、照射后干细胞治疗的比格犬的临床特点、肠镜表现、生存时间,并采用组织病理学评分对放射造成的肠损伤程度进行评估。结果比格犬肠损伤程度与放射剂量呈正相关。E、F组干细胞治疗后肠损伤组织病理学评分明显低于治疗前(P<0.05);G组治疗前后比较,差异无统计学意义(P>0.05)。比格犬生存时间与放射剂量呈负相关。F组生存时间明显长于C组(P<0.05);E组与B组、G组与D组比较,差异无统计学意义(P>0.05)。结论随着放射剂量增大,比格犬急性放射性肠病加重,生存时间缩短。骨髓间充质干细胞移植在12 Gy剂量的急性放射性肠病的治疗中发挥积极作用。

Autologous bone marrow stem cells transplantation treatment in acute radiation enteritis of Beagle dogs

Objective To investigate the clinical effect of autologous bone marrow stem cells transplantation treatment in acute radia-tion enteritis of Beagle dogs through the establishment of acute radiation enteritis damage model. Methods A total of 35 Beagle dogs were randomly divided into 7 groups(from Group A to Group G)and there were 5 dogs in each group:the control group(Group A), 10 Gy single exposure group( Group B) ,12 Gy single exposure group( Group C) ,14 Gy single exposure group( Group D) ,10 Gy expo-sure combined MSCs transplantation group( Group E) ,12 Gy exposure combined MSCs transplantation group( Group F) and 14 Gy ex-posure combined MSCs transplantation group(Group G). X-ray intensity modulated radiation was carried out on abdomen of Beagle dogs,the clinical features,colonoscopy results and survival time were observed after single exposure,exposure combined MSCs trans-plantation;the degree of intestinal tract injury after exposure were evaluated using histopathologic score. Results There was a positive correlation between degree of the intestinal mucosal injury and the radiation dose. In Group E and Group F,the histopathologic score after MSCs transplantation were significantly lower than that before MSCs treatment(P<0. 05);there was no statistical significance be-tween before and after treatment in Group G ( P>0. 05 ) . There was a negative correlation between the survival time and radiation dose. The survival time was significantly longer than that in Group C(P<0. 05);there were no statistical significance in the compari-son between Group B and Group E,Group D and Group E(P>0. 05). Conclusion Along with the increase of radiation dose,acute radiation enteritis in Beagle dogs is more serious,and the survival time is shorter. Autologous bone marrow stem cells transplantation plays a positive role in curing acute radiation enteritis in Beagle dogs with a dose of 12 Gy.