补阳还五汤对局灶性脑缺血大鼠血管新生的影响

目的 探讨补阳还五汤对局灶性脑缺血大鼠血管新生的影响.方法 采用大脑中动脉线栓法建立右侧局灶性脑缺血大鼠模型,将动物随机分为正常组、假手术组、模型组、中药组、重组人血管内皮抑制素(ED)组和中药+ED组,腹腔注射5-溴脱氧尿嘧啶核苷,给予不同处理,分别于1、7、14天和28天处死动物,采用免疫组织化学法观察血管新生情况.结果 正常脑内存在一定的5-溴脱氧尿嘧啶核苷、血管性血友病因子阳性细胞,脑缺血后表达迅速增加,7天达高峰,然后逐渐下降;ED组5-溴脱氧尿嘧啶核苷、血管性血友病因子阳性细胞数量明显少于同期各组(P<0.05);补阳还五汤可显著增强5-溴脱氧尿嘧啶核苷、血管性血友病因子的表达,在第7天和14天时明显多于其它各组(P<0.05),7天时补阳还五汤+ED组5-溴脱氧尿嘧啶核苷、血管性血友病因子阳性细胞数多于模型组和ED组(P<0.05),但少于补阳还五汤组(P<0.05).用药7天和14天,补阳还五汤组降低神经功能积分作用与其它用药组比较差异有统计学意义(P<0.05).补阳还五汤+ED组降低积分作用强于模型组和ED组(P<0.05),但较补阳还五汤组作用弱(P<0.05).结论 补阳还五汤可促进局灶性脑缺血大鼠缺血区血管新生,并可部分拮抗重组人血管内皮抑制素阻断的血管新生,提示其可能通过其他途径影响血管新生.

Effect of Buyang Huanwu Decoction on Angiogenesis in Rats after Focal Cerebral Ischemia

Aim To explore the effect of Buyanghuanwu decoction(BYHWD)on angiogenesis in rats after focal cerebral ischemia.Methods The model of focal cerebral ischemia in rats was reproduced by middle cerebral artery occlusion.Rats were randomly divided into navie,shame-operated,ischemia,Buyanghuanwu decoction(BYHWD),ED and BYHWD+ED group.Animals were received BrdU-labeled and killed at 1,7,14 and 28 day point after operation.BrdU and vWF were measured by immunohistochemical staining.Results There was a certain BrdU or vWF positive cells in normal brain,after cerebral ischemia BrdU positive cells were increased and reached the peak at 7 day.The expression of BrdU or positive cells of ED group were less than the others remarkably at the same time point(P<0.05).BYHWD can significantly increase the expression of BrdU or vWF.The increase was statistically significant at 7 and 14 day point after ischemia(P<0.05).The expression of BYHWD+ED groups was more than model and ED group at 7 day time point(P<0.05),but less than BYHWD group(P<0.05).Compared to the other groups,there was statistical significance on BYHWD for decreasing the number of nerve function at 7 and 14 day points(P<0.05).The effection of decreasing nerve function number on BYHWD+ED was better than model and ED groups(P<0.05),but less than BYHWD(P<0.05).Conclusion BYHWD may promote the angiogenesis in rats after focal cerebral ischemia.BYHWD can partly promote the angiogenesis which ED restrained.The result shows that it affects ischemia by other ways.