雷帕霉素滴眼液对碱烧伤角膜新生血管的影响

目的探讨雷帕霉素（Rapamycin）滴眼液对兔碱烧伤后角膜新生血管的抑制作用。方法48只新西兰白兔右眼碱烧伤制成新生血管模型，随机分成A组（0．1％雷帕霉素滴眼液组），B组（0．5％雷帕霉素滴眼液组），C组（1％雷帕霉素滴眼液组），D组（赋形剂对照组）。烧伤后每天滴眼4次。观测角膜浑浊、融解情况和新生血管生长并测量长度。角膜组织HE染色观察病理改变，炎性细胞计数，免疫组化检测角膜内皮生长因子（VEGF）的表达。结果碱烧伤后各组角膜均出现不同程度的角膜浑浊、融解，随时间延长，对照组角膜浑浊、融解明显，角膜基质内大量炎性细胞浸润和粗大新生血管形成。治疗组的角膜浑浊、融解减轻，伤后7d、14dB、C组炎症细胞计数、角膜新生血管面积及VEGF表达均小于对照组（P〈0．05）。各组角膜新生血管面积与角膜炎性细胞数及VEGF表达呈正相关。结论0．5％及1％雷帕霉素滴眼液可有效抑制碱烧伤引起的角膜免疫炎症反应，抑制角膜新生血管的生长，为眼碱烧伤后角膜新生血管的治疗提供了新的选择。

Effect of rapamycin applied locally in corneal neovascularization of rabbit after alkali burning

Objective To investigate the efficiency of corneal alkali burn treating with rapamycin. Methods The corneal neovascularization was induced by alkali burn in the right eyes of 48 New Zealand white rabbits. The rabbits were randomly divided into 4 groups,0.1% rapamycin in group A,0.5% rapamycin in group B, 1% rapamycin in group C, excipient in group D, four times daily in every group. The Inflammation of corneas were observed, the lengths and areas of corneal angiogenesis were measured. Corneas were analyzed by H-E staining and inflammatory cells were evaluated. Immunohistochemistry were used to determine VEGF protein level. Results Inflammation in control group was obvious,massive inflammatory ceils invasion and neovascularization were observed. In treatment group, the corneal stroma inflammation was inhibited in some extent,inflammatory cells,the areas of neovascularization,the level of VEGF protein in group B, C were fewer than those in control group （P 〈0.05 ）. The area of CNV had close relationship with the number of inflammatory cells and VEGF protein level （P 〈0.05 ）. Conclusion Topical application of 0.5% and 1% rapamycin has significant effects on theinhibition of corneal neovascularization.