清热利湿法对慢性乙型肝炎HBV基因突变株复制的影响

目的:研究清热利湿法对慢性乙型肝炎(CHB)患者HBV前C区基因突变株复制的影响。方法:采用基因芯片技术定量检测CHB肝胆湿热/湿热中阻证患者治疗(清热利湿法)前后的HBV前C基因变异。结果:治疗前的HBV前C区1762、1764、1896位点变异率明显高于1899位点变异率,差异有显著性意义(P<0.01);治疗后各位点变异率下降,其中治疗组1764、1896位点变异率显著下降(P<0.05)。治疗前不同位点变异株信号强度1896>1764>1762>1899,差异具有显著性意义(P<0.05);治疗后1762、1764、1896位点变异株信号强度下降,其中1896位点信号强度显著下降(P<0.05)。结论:清热利湿法在改善CHB肝胆湿热/湿热中阻证患者临床证候的同时,可在一定程度上抑制HBV前C区突变株的复制。

The clearing heat and promoting diuresis therapy afect the gen copy of the precore gent of HBV mutative strains in chronic hepatitis B patients

Objective:To research the affect of clearing heat and promoting diuresis therapy on copy of the precore gene with hepatitis B virus mutative strains in chronic hepatitis B patients.Methods:The precore gene mutation was detected by gene chip with guantity in chronic hepatitis B patients with heat and dampness syndrome in liver-gallbladder.Results:The mutative rate of 1762,1764 and 1896 sites were apparently higher than 1899 site before and after treatment,and the difference was obvious(P<0.01).Mutative rate of every site was came down after treatment,and mutative rate of 1764 and 1896 site in treated team were obviously decreased(P<0.05).The signal intensity of mutative strains in different site had obviously difference before treatment(P<0.05),1896>1764>1762>1899.The signal intensity of 1762,1764,1896 site was reduced after treatment,and the intensity of 1 896 site noticeably decreased(P<0.05).Conclusion:Clearing heat and promoting diuresis therapy could depress copy of the precore gene of hepatitis B virus mutative strains in a certain extent,during relieving clinical symptoms of patients with heat and dampnes syndrome in liver-gallbladder.