| 应用高速逆流色谱技术从延胡索中分离制备延胡索乙素 |
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| 引用本文： | 俞雁,金艳,徐超,沈平孃,张继全. 应用高速逆流色谱技术从延胡索中分离制备延胡索乙素[J]. 世界科学技术, 2006, 8(2): 17-19,5 |
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| 作者姓名： | 俞雁 金艳 徐超 沈平孃 张继全 |
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| 作者单位： | [1]华东理工大学化学与制药学院,上海200237 [2]国家中药制药工程技术研究中心,上海201203 |
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| 摘 要： | 利用高速逆流色谱技术,一次进柱过程即可从延胡索粗提物中分离到活性成分延胡索乙素.两相溶剂系统用石油醚-乙酸乙酯-甲醇-水四元系统,上相为固定相,下相为流动相,转速为850r/min,流速为1.2ml/min.通过EI-MS和1H-NMR鉴定,基本确认了其化学结构.经HPLC分析,纯度达96.4%.
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| 关 键 词： | 高速逆流色谱 延胡索 延胡索乙素 |
| 收稿时间： | 2005-10-19 |
| 修稿时间： | 2005-10-192005-11-17 |
Separation and Preparation of dl-tetrahydropalmatine from Crude Extract of Corydalis Yanhusuo W.T. Wang by High-s peed Countfercurrent Chromatography Technology |
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| Yu Yan,Jin Yan,Cu Chao,Shen Pinniang,Zhang Jiquan. Separation and Preparation of dl-tetrahydropalmatine from Crude Extract of Corydalis Yanhusuo W.T. Wang by High-s peed Countfercurrent Chromatography Technology[J]. World Science and Technology(Modernization of Traditional Chinese Medicine), 2006, 8(2): 17-19,5 |
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| Authors: | Yu Yan Jin Yan Cu Chao Shen Pinniang Zhang Jiquan |
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| Affiliation: | 1. East China University of Science and Technology, Shanghai 200237;2.National Engineering Research Center for Traditional Chinese Medicine,Shanghai 201203 |
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| Abstract: | The active components of dl-tetarohydropalmatine can be separated from the crude extract of corydalis yanhusuo W.T.Wang in the process of one-step elution by the technology of high-speed countercurrent chromatography.The binary system of its solvents consists of petroleum ether-ethyl acetate-methanol-the quarternary system of water with the stationary phase as its upper layer and the mobile phase as its lower layer,850r/min as the operational speed of the instrument used and 1.2ml/min as the flow speed of the solvents.The chemical structure of the separated dl-tetrahy dropalmatine is confirrnned through the identification of EI-MS and IH-NMR and its purity reaches 96.4% by HPLC analysis. |
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| Keywords: | high-speed countercurrent Chromatography.Corydalis yanhusuo W.T.Wang dl-tetrahydropalmatine |
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