口腔癌TIL的表型及细胞毒活性测定

作者在成功地建立了口腔癌TIL的分离、培养方法的基础上,采用单抗致敏的红细胞花环试验和H~3-TDR释放法,对10例口腔癌TIL的表型及功能特征进行了动态分析。结果发现新分离TIL中CD_3~ 、CD_4~ 、CD_8~ 较低,体外培养后,TIL中各亚群均有增加,但以CD_3~ 、CD_8~ 增加为主;新分离TIL的NK和LAK活性分别为10％和10.5％,随体外培养时间延长,其细胞毒活性增强,在4周时达高峰。NK和LAK活性分别为50％和44.8％。4周后,其活性开始降低。提示口腔癌TIL的临床应用时机为培养4周左右。

Analyses on phenotypes and cytotoxicity of TILs from patients with oral cancer

With the methods of rosette test and H3 - TDR release assay respectively, We analysed phenotypes and cytotoxicity of TILs obtained from patient with oral cancer. The results showed that,CD3 CD4 and CD8 lymphocytes scattered in the newly isolated TILs, cultured with low - concentration of rIL-2 in vitro,TILs expressed CD3 and CDg predominantly, though CD4 cells increased significantly. Their cytotoxicity against both NK - sensitive K562 and LAK - sensitive Raji targets were limited before cultivation(only10.0% and 10.5% respectively) .increased along with the time of proliferation and reached the peak(up to 50.0% and 44.8% respectively)at the 4th culture- week. This study demonstrated that the clinical transfer of TILs is administrated after four - week incubation in vitro.