单纯疱疹病毒性脑炎小鼠脑组织中IL-10的表达

目的通过检测白细胞介素10（IL-10）在单纯疱疹病毒性脑炎（HSE）小鼠脑组织中的表达，探讨IL-10在HSE发病中可能的免疫学机制。方法采用颅内接种法建立HSE小鼠模型。设置正常对照组、病毒感染组、阿昔洛韦组和地塞米松组。通过神经症状评分观察HSE小鼠的发病情况，应用免疫组化法测定各组小鼠脑组织中IL10的表达，并采用Pearson相关分析分析神经症状评分与IL-10表达的相关性。结果病毒感染组神经症状评分最高，生存率最低，阿昔洛韦组次之，地塞米松组神经症状评分最低，生存率最高（F=30．20，x^2=23．20，P〈0．05）。IL-10在HSE小鼠脑组织中的表达较正常对照组明显上调，病毒感染组较阿昔洛韦组增高，病毒感染组和阿昔洛韦组较地塞米松组增高（F-10．65，P〈0．05）。神经症状评分与IL-10的表达呈正相关（r=0．83，P〈0．05）。结论IL-10可能通过抑制小胶质细胞炎性因子的产生，减轻神经细胞损伤等介导宿主在HSE中的免疫应答作用。

IL-10 EXPRESSION IN BRAIN TISSUE OF MICE WITH HERPES SIMPLEX VIRUS ENCEPHALITIS

Objective To study the IL-10 expression in brain tissue of mice with herpes simplex encephalitis （HSE） and explore the possible immunologic mechanism of IL-I0 in the onset of this disease. Methods A mouse model of HSE was created by intracranial innoculation. Four groups-normal control group, viral infection group, acyclovir group, and dexamethasone group- were set up. The incidence of the onset of HSE mice was observed through the score of neurologic symptoms, the IL-10 expression was detected by immunohistochemistry, and the correlation between symptomatic score and the expression of IL-10 was analyzed by using Pearson Correlations. Results The highest neurological symptomatic score was recorded in viral-infection group, with the lowest survival rate, the next was aeyclovir-treated group, the lowest neurologic symptomatic score was noted in dexametha sone treated group, with the highest survival rate （F=30. 20, x^2= 23. 20, P〈0.05）. The expression of ILl0 in brain tissue of HSE mice was higher than that in the normal control, that in viral infection group was higher than that in acyclovir treated group, that in both viral infection and aeyclovir treated group were higher than that in dexamethasone-treated （F=10.65,P〈0.05）. IL-10 was positively correlated with neurologic symptomatic score （r 0.83,P%0.05）. Conclusion IL-IO may inhibit the produc tion of inflammatory factor of gitter cells, and lighten the immune injury to regulate the size of immune response.