多发性骨髓瘤病人血清sgp130与IL-6变化及意义

目的探讨多发性骨髓瘤（MM）病人血清可溶性糖蛋白130（sgp130）和白细胞介素6（IL-6）的动态变化及临床意义。方法采用双抗体夹心酶联免疫吸附（ELISA）法,检测60例MM病人和20例健康体检者（对照组）血清sgp130及IL-6的水平,并观察二者与血清β2-微球蛋白（β2-MG）水平的相关性。结果 MM病人血清sgp130、IL-6水平均明显升高,与对照组比较差异有显著性（t=5.51、8.29,P〈0.01）,并随MM临床分期增加、病情加重而逐渐升高（F=23.21、21.86,P〈0.05）,但不同免疫亚型间差异无显著性（F=7.37、4.12,P〉0.05）。血清sgp130、IL-6水平的变化与化疗效果有关,化疗有效病人血清sgp130、IL-6水平均明显下降,其中BD组（硼替佐米＋地塞米松）下降程度明显大于VAD组（长春地辛＋表柔比星＋地塞米松）,差异有显著性（t=11.77、5.31,P〈0.05）。血清sgp130、IL-6水平与血清β2-MG水平呈正相关（r=0.683、0.549,P〈0.01）。结论血清sgp130及IL-6水平变化与MM的肿瘤负荷和化疗效果有关,可作为评估病情变化和治疗效果的有效指标。

CHANGES AND CLINICAL SIGNIFICANCE OF SERUM SOLUBLE GLYCOPROTEIN-130 AND INTERLEUKIN-6 IN PATIENTS WITH MULTIPLE MYELOMA

Objective To investigate the changes of serum soluble glycoprotein-130 （sgpl30） and interleukin-6 （IL-6） in patients with multiple myeloma （MM） and the clinical significance. Methods By using enzyme-linked immunosorbent assay （ELISA）, serum sgp130 and IL-6 were measured in 60 patients with MM and 20 healthy controls. The above two parameters were compared with β2-microglobulin on their correlation. Results The serum levels of both sgpl30 and IL-6 in MM patients were obviously elevated, as compared with the control, the difference was significant （t= 5.51,8.29;P〈0.01）, which were increased along with the increase of clinical stage of MM and the severity of the disease （F= 23.21,21.86;P〈0.05）, but the difference be- tween different isoforms was not significant （F= 7.37,4.12;P〉0.05）. The changes of serum levels of sgpl30 and IL-6 were re- lated with chemotherapeutic effectiveness. In patients who responded well to the therapy, the levels of both sgpl30 and IL-6 de- scended dramatically, in which, the decrease of that in bortezomib-dexamethasone group was greater than that in vindesine-epirubi ein-dexamethasone group （t=11.77,5, 31 ;P〈0.05）. The levels of sgp130 and IL-6 were positively correlated with β2-microglobu- lin level （r=0. 683,0. 549 ;P〈0.01）. Conclusion The changes of serum levels of sgp130 and IL-6 were related to tumor load and efficacy of chemotherapy, which can be used as an effective index to assess a patient＇s condition and the outcome of treatment.