| EL一4细胞凋亡与细胞周期的辐射效应 |
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| 引用本文： | 张迎春,苏旭,刘建香,刘树铮.EL一4细胞凋亡与细胞周期的辐射效应[J].吉林大学学报(医学版),1995(5). |
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| 作者姓名： | 张迎春 苏旭 刘建香 刘树铮 |
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| 作者单位： | 白求恩医大卫生部放射生物实验室 |
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| 摘 要： | 采用流式细胞术（ＦＣＭ）研究了０．５、１、２、４ＧｙＸ射线照射后，小鼠淋巴瘤细胞ＥＬ一４的凋亡与细胞周期的剂量效应关系。结果表明：以上剂量照射后２４ｈ，Ｇ１期细胞呈剂量依赖性增加，出现明显的Ｇ１阻滞；而Ｓ期细胞则剂量依赖性减少；Ｇ２期出现明显的Ｇ２阻滞；细胞凋亡亦呈剂量依赖性增力。该结果提示：ＥＬ一４细胞经较高剂量ｘ射线照射后，出现明显的ＤＮＡ合成抑制和Ｇ２阻滞，致使ＥＬ一４细胞的有丝分裂延迟。统计分析表明：Ｇ１阻滞与细胞凋亡高度相关ｒ＝０．９１。
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| 关 键 词： | EL一4细胞，流式细胞仪（FCM），细胞周期，细胞凋亡，X射线 |
Radiation effects of EL一4 cells on their apoptosis and cell cycle progression |
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| Zhang
Yingchun,Su Xu,Liu Jianxiang,et al.Radiation effects of EL一4 cells on their apoptosis and cell cycle progression[J].Journal of Jilin University: Med Ed,1995(5). |
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| Authors: | Zhang
Yingchun Su Xu Liu Jianxiang |
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| Abstract: | The present paper described the radiation effect of EL一4 on the cell cycle
progressionand apoptosis by flow cytometic analysis after staining with propidium iodide. It
was found that 24hoturs after 0.5、1.0、2.0、4. 0 Gy X一irradiation, the cells of G1 phase
showed dose一dependent in-crease;the significant G1 arrest was shown in Gl phase; the cells
of S phase showed dose一dependentdecrease; significant G2 block appeared in G2
phase;apoptosis also showed the dose一dependent in-crease. The results indicated that after
high dose X一irradiation, the mitosis of EL一4 was delayedwith significant suppression of DNA
synthesis and G2 block. G1 arrest highly correlated with apopto-sis,the correlation coefficients
being 0, 91. |
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| Keywords: | EL一4 cells apoptosis cell cycle radiation |
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