负载胃癌干细胞抗原的树突细胞对胃癌细胞免疫作用的研究

目的探讨胃癌干细胞抗原负载树突细胞(DC)联合细胞因子诱导杀伤细胞(CIK)对胃癌细胞的影响。方法分离人胃癌干细胞,冻融法制备抗原,将胃癌干细胞负载DC-CIK细胞,用流式细胞仪检测胃癌干细胞和DC、CIK细胞表型;将胃癌细胞与DC组、DC-CIK组、胃癌细胞抗原组、负载胃癌干细胞抗原组联合培养,用四甲基偶氮唑蓝法(MTT)检测不同组别对胃癌细胞的杀伤作用。结果经胃癌干细胞抗原刺激的DC细胞,DC表面成熟标志CD83和CD86表达明显增加,CD83和CD86表达率为80.4%,高于DC组、DC-CIK组、胃癌细胞抗原组,差异有统计学意义(P<0.01);MTT检测结果显示:负载胃癌干细胞抗原组、胃癌细胞抗原组、DC-CIK组、DC组对胃癌细胞杀伤率分别为(80.6±0.8)%、(72.3±0.6)%、(58.4±0.2)%和(49.7±0.8)%,差异有统计学意义(P<0.01)。结论胃癌干细胞作为抗原刺激DC细胞,可增强树突状细胞免疫原形表达,促进细胞增殖,提高对胃癌细胞的杀伤作用。

A study of the immune function of DC-CIK loaded with gastric cancer stem cell antigen against the activity of gastric cancer

【Objective】 This study is aimed at investigating the killing effect of dendritic cells（DC） and cytokine-induced killer（CIK） cells loaded with gastric cancer stem cell antigens against gastric cancer cells.【Methods】Human gastric cancer stem cells are Isolated and identified.Antigens are made with the freeze-thaw method.We load gastric cancer stem cells to DC-CIK cells and examine the DC cell phenotype with flow cytometry.Methyl thiazolyl tetrazolium（MTT）method was used to examine the killing effect of DC-CIK on gastric cancer cells.【Resluts】Gastric cancer stem cell antigen-stimulated DC cells the DC surface maturity logo CD83 and CD86 expression significantly increased,CD83 and CD86 expression was 80.4%,higher than the DC group,DC-CIK group,gastric cancer cell antigen group,（P＆lt;0.01） differences;MTT test results show that：the destruction rate of gastric cancer cell destruction,load gastric cancer stem cell antigen group,gastric cancer cell antigen group,DC-CIK group,DC group were（80.6±0.8）%,（72.3±0.6）% was statistically significant;（58.4±0.2）% and（49.7±0.8）%（P＆lt;0.01）,the difference was statistically significant.【Conclusion】Stimulating DC cells with gastric cancer stem cells as an antigen can enhance the immunoreaction,promote cell proliferation,and therefore improve killing effect on gastric cancer cells.