苏云金杆菌的急性毒性及致敏实验观察

按照《农药登记毒理学试验方法》(GB15670-1995),对生物衣药苏云金杆菌原药进行急性经口、经皮、吸入毒性试验,眼睛刺激试验和致敏试验。该受试物对雄、雌性大鼠急性经口LD50为3830、3160 mg／kg;对雄、雌性大鼠急性经皮LD50(4 h)>2150 mg／kg;对雄、雌性大鼠急性吸入LC50(2 h)>5 000 mg／m3;对实验兔急性眼平均刺激指数为0;对豚鼠致敏试验,致敏率为0％。因此,该受试物急性经口属微毒性、急性经皮属低毒性、急性吸入属微毒性,眼睛刺激试验为无刺激性,致敏试验属Ⅰ级弱致敏物。

Acute Toxicity and Dermal Sensitzation of Bacillus thuringiensis Original Drug

According to toxicological test methods of pesticides for registration(GB15670-1995),The test of acute toxicity of biopesticide - bacillus thuringiensis, include acute oral toxicity, acute dermal toxicity, acute inhalation toxicity, acute eye irritation test and dermal sensitization were carried out. The acute oral toxicity LD50 of this biopesticide was 3830 mg/kg in male and 3160 mg/kg in female, acute dermal toxicity LD50(4h)>2 150 mg/kg, LC50(2h)>5 000 mg/m3, there is no irritation to rabbit eye, dermal sensitization : rate of sensitization is zero.So the acute oral toxicity , acute dermal toxicity and acute inhalation toxicity of bacillus thuringiensis are lower. There is no irritation to rabbit eye, and it belongs to grade I weak sensitizing matter.