先兆子痫患者蛋白 Z 水平及其内含子 FG79A 基因多态性的实验研究简

目的探讨先兆子痫患者血浆蛋白z（PZ）水平及其内含子FG79A基因多态性的发生率与先兆子痫之间的相关性和临床意义。方法对先兆子痫组160例和正常妊娠对照组162例检测PZ水平并采用PCR和限制性内切酶片段长度多态性方法并结合基因测序检测PZ内含子FG79A基因多态性，同时检测两组D-D、FDP、AT：A的水平，观察两组各项指标变化情况。结果先兆子痫组PZ水平显著低于正常妊娠对照组（1．57±0．32mg／Lvs2．12±0．35mg/L，P〈0．05）；两组人群均存在PZ内含子FG79A基因多态性。先兆子痫组GG型、GA型和AA型分别占19．38％、53．75％和26．87％，G、A等位基因频率分别为46．25％和53．75％；正常妊娠对照组GG型、GA型和AA型分别占17．90％、54．94％和27．16％，G、A等位基因频率分别为45．37％和54．63％，两组基因型和等位基因频率分布无显著差异（均P〉0．05）；先兆子痫组D-D、FDP水平明显高于正常对照组（5．15±1．03μg／ml vs0．58±1．32μg/ml，P〈0．05；19．46±3．28μg/ml vs 2．93±1．92μg/ml，P〈0．05），而AT：A水平无显著差异（99．12±13．5％vs100．8±10．6％，P〉0．05）。结论先兆子痫患者存在PZ内含子FG79A基因多态性，但该基因多态性与疾病的发生关联不大，然而该疾病与Pz水平、D-D、FDP水平却有一定的相关性。

The Clinical Research of Plasma Protein Z Level and PZ Intron FG79A Polymorphisms with Pre- eclampsia

Objective To investigate the level of PZ and the distribution of PZ intron F G79A polymor- phisms in patients of preeclampsia and clinical significance. Method The test group was consisted of 160 pa- tients of preeclampsia, the control group was consisted of 162 women with normal pregnancy. The PZ intron F G79A polymorphisms were studied by PCR and restriction fragment length polymorphism. Meanwhile, D-D, FDP, AT Ⅲ：A and PZ level were tested. Observing changes in two groups. Results The level of PZ in test group was lower than that in control group（ 1.57 ±0. 32 mg/L vs 2. 12 ± 0. 35 mg/L,P 〈 0. 05 ）. The PZ in- tron F G79A polymorphisms were found in two groups. The percentages of GG/GA/AA were 19.38%/ 53.75%/26. 87% in test group. The frequencies of G and A alleles were 46.25% and 53.75%. The percent- ages of GG/GA/AA were 17.90%/54.94%/27.16% in control group. The frequencies of G and A alleles were 45.37% and 54.63%. There were no significant difference in PZ intron FG79A genotype and alleles fre- quencies distribution between test group and control（ P 〉 0. 05 ）. The levels of D-D and FDP in test group were higher than those in control group （ 5.15 ± 1.03 μg/ml vs 0. 58 ± 1.32 μg/ml, P 〈 0. 05 ） ; （ 19.46 ± 3.28 μg/ml vs 2.93 ± 1.92 μg/ml,P 〈0.05） ,there was no difference in ATm ：A between two groups（99.12 ± 13.5 % vs 100. 8 ± 10. 6%, P 〉 0. 05 ）. Conclusion PZ intron F G79A polymorphisms are existed in pa- tients of preelampsia,but its polymorphisms were not associated with the disease. While the decreased PZ lev- el, increased D-D and FDP levels may be associated with the preeclampsia.