软脉灵药物血清抑制PDGF诱导的平滑肌细胞迁移研究

目的观察软脉灵药物血清对血小板源性生长因子（PDGF）诱导的大鼠血管平滑肌细胞（VSMCs）迁移的影响。方法采用体外组织贴块法培养大鼠胸主动脉血管平滑肌细胞；应用灌胃法制备SD大鼠的软脉灵药物血清；应用改良Boyden微孔膜双槽法进行细胞迁移实验；用FITC-鬼笔环肽标记F—actin，并在激光共聚焦显微镜下观察PDGF诱导的VSMCs骨架形态的变化。结果含软脉灵药物血清可显著抑制PDGF—BB诱导的VSMCs迁移，呈现一定的浓度依赖性，10％药物浓度时抑制作用最明显，抑制率为61．42％（P〈0．01）。PDGF（10ng／mL）刺激后细胞即发生伸展，应力纤维增多，可见伪足形成；加入含软脉灵药物血清后可见到伪足及张力纤维减少，而且随着软脉灵浓度的升高，可见到伪足及张力纤维显著减少。结论软脉灵药物血清能显著抑制PDGF—BB诱导的VSMCs迁移和应力纤维形成。

Effect of Ruanmailing Decoction Drug containing Serum on the Migration Induced by Platelet Derived Growth Factor in Vascular Smooth Muscle Cells

Objective To investigate the effect of Ruanmailing decoction drug containing serum on the migration induced by platelet derived growth factor （PDGF） in vascular smooth muscle cells （VSMCs） from rats. Methods Cultured VSMCs were derived from rat thoracic aorta. VSMCs were identified by smooth muscle actin monoclonal antibody. Ruanmailing decoction drug containing serum was prepared from rats after 5 days of drug garage. Cell migration was determined by modified Boyden chamber assays. The F actin polymerization was detected by FITC phalloidine labeling and examined by confocal microscope. Results Ruanmailing decoction drug containing serum produced an inhibition of the migration induced by PDGF BB, the peak inhibition rate was 61.42%（P〈0.01）. PDGF（10 ng/mL） stimulated a increase in stress fiber and pseudopods formation. Pseudopods and tension fiber were sig nificantly decreased in the presence of Ruanmaiting decoction drug containing serum, which represented a concentration depend manner. Conclusion Ruanmailing decoction drug containing serum could significantly inhibit the migration of VSMCs and cell skele tal reorganization induced by PDGF.