加味温胆汤对抑郁模型大鼠海马NMDA—NR1mRNA／蛋白表达的影响

目的探讨加味温胆汤对抑郁模型大鼠海马NMDA—NR1mRNA／蛋白表达的影响作用。方法以孤养结合慢性不可预见性应激抑郁模型大鼠为研究对象，在实验7d、14d、21d、28d时，检测各组大鼠旷场实验中水平运动、垂直运动得分，并采用QRT—PCR、Western—Blot技术检测各组大鼠海马NMDA—NR1mRNA／蛋白表达变化。结果21d、28d时，与空白组比较，模型组水平运动、垂直运动得分降低（P〈0．01）；与模型组比较，中、西药组水平运动、垂直运动得分增加（P〈0．05或P〈0．01）。21d、28d时，与空白组比较，模型组大鼠海马NMDA—NR1mRNA／蛋白表达增多（P〈0．01）；与模型组比较，中、西药组大鼠海马NM—DA—NR1tuRNA／蛋白表达减少（P〈0．05或P〈0．01）。结论加味温胆汤的抗抑郁效应可通过阻抑海马NMDA—NR1mRNA／蛋白表达而实现。

The Influence of Modified Wendan Decoction on NMDA- NR1 mRNA/protein Expression in Hippocampus in Rat Model with Depression

Objective To explore the effect of Modified Wendan decoction on N - methyl - d - aspartic acid （NMDA）- NR1 mR- NA/protein expression in the hippocampus of the rat model with depression. Methods The rat models with unpredictable chronic stress and isolation - induced depression were studied. Models were randomly divided into four groups and the rats of each group were intragastrically perfused with corresponding interventions at the day 7th, 14 th, 21 st, and 28 th. The behaviors of open -field test were examined and the NMDA - NR1 mRNA/protein expression in the hippocampus was tested by the method of QRT - PCR and Western - Blot. Results At the day 21st,28th,the score of vertical motion and horizontal motion behaviors of open - field test in model group was lower than that in blank group （P〈0.01）. The score of vertical motion and horizontal motion behaviors of open - field test in Chinese medicine group and western medicine group was higher than that in model group （P〈0.05 or P〈0.01）. At the day 21st and 28th,The expression of NMDA- NR1 mRNA/protein in the hippocampus of the rats in model group were higher than that in blank group （P〈0.01）. The expression of NMDA - NR1 mRNA/protein in the hippocampus of the rats in Chinese medicine group and western medicine group were lower than that in model group （P〈0.05 or P〈0.01）. Conclusion The mechanism of the anti - depressant effect of Modified Wendan Decoction is related to down - regulate the expression of NMDA NR1 mRNA/protein in the hippocampus of depression.