| 高效液相色谱法测定不同色泽荜茇中胡椒碱含量 |
| |
| 引用本文： | 李普泉,白音夫. 高效液相色谱法测定不同色泽荜茇中胡椒碱含量[J]. 中国民族医药杂志, 2009, 15(5): 44-45 |
| |
| 作者姓名： | 李普泉 白音夫 |
| |
| 作者单位： | 1. 内蒙古自治区中蒙医医院,呼和浩特,010020
2. 内蒙古自治区中蒙医研究所,呼和浩特,010020 |
| |
| 摘 要： | 目的：采用HPLC方法测定荜茇灰色、黑色、棕色胡椒碱含量。方法：色谱柱Q（4．6×150mm，5μm）；流动相：甲醇-水（77：23）；流速：1mL·min^-1；柱温30℃；测定波长：343nm。结果：胡椒碱9．2～46．0μg·mL^-1成线性关系（r=0．999）；回收率为100．4％；RSD=2．5％；荜茇灰色、黑色、棕色胡椒碱含量分别为15．00、16．35、10．28mg·g^-1。结论：灰色、黑色荜茇胡椒碱含量高于棕色荜茇胡椒碱含量。
|
| 关 键 词： | 荜茇 胡椒碱 高效液相色谱 |
HPLC determination of piperine in different color Piper longum L |
| |
| LI Pu-quan,BAI YINFU. HPLC determination of piperine in different color Piper longum L[J]. Journal of Medicine & Pharmacy of Chinese Minorities, 2009, 15(5): 44-45 |
| |
| Authors: | LI Pu-quan BAI YINFU |
| |
| Affiliation: | LI Pu -quan, BAIYINFU (Hospital of Inner Mongolia Chinese - Mongolin traditional Medicine 010020 Huhhte ;Institute of Inner Mongolia Chinese- Mongolia traditinal Medicine 010020 Huhhte) |
| |
| Abstract: | Objective： to determinate piperine in different color Piper longum L. （black, grey and brown）. Methods： C18 column （4.6 × 150mm,5μm） with a mobile phase consisting of method- water（77：33）.The flow rate was set at 1ml· mL^-1, the column temperature was 30℃ and detected by UV 343nm. Results： Within 9.2- 46.0μg· mL^-1 Piperine had a good linearity,r = 0. 999. The average recovery was 100.4%,RSD = 2.5%,Piperine was 15.00,16.35,10. 28mg· g^-1 in black, grey and brown Piper longum L respectively. Conclusion： Piperine in black and greyand Piper longum L were higher than brown. |
| |
| Keywords: | Piper longum L Piperine HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
