Maternal ethanol consumption: binding of L-glutamate to synaptic membranes from whole brain, cortices, and cerebella of offspring |
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| Authors: | G M Kelly M J Druse D A Tonetti B G Oden |
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| Institution: | 1. Department of Pathology and Rogel Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA;2. Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA;3. Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan;4. CIIL-Centre d’Infection et d’Immunité de Lille, Université de Lille, CNRS, Inserm, CHRU Lille, Institut Pasteur de Lille, U1019-UMR 8204, F-59000, Lille, France;5. Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA;1. Frick Chemistry Laboratory, Department of Chemistry, Princeton University, Washington Road, Princeton, NJ 08544, USA;2. Graduate Program, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA;1. Wroclaw University of Science and Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland;2. University of Gdansk, Faculty of Chemistry, Wita Stwosza 63, 80-308, Gdansk, Poland;3. INSERM U-1100, “Centre d’Etude des Pathologies Respiratoires” and Université François Rabelais, 37032, Tours, France;1. Department of Pharmacology, Columbia University, New York, NY 10032, USA;2. Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA;3. Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile;1. Deparment of Pediatrics, College of Physicians & Surgeons, Columbia University, New York, NY 10032, USA;2. Department of Microbiology & Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15260, USA |
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| Abstract: | We examined the influence of chronic maternal ethanol consumption on the Na+- and Ca2+-independent binding of L-glutamate to synaptic plasma membranes from whole brain as well as from cortices and cerebella of developing offspring. The maximum specific binding (Bmax) of L-glutamate to the Na+- and Ca2+-independent binding sites in synaptic plasma membranes of brain peaked at 17 days of age in the offspring of both control and ethanol-fed rats, although at that age there were significantly fewer binding sites in the brains of the offspring of ethanol-fed rats. The regional localization of this deficit is not now known. However, it appears that one major glutamatergic region (the cortex) does not reflect the transient deficiency of L-glutamate sites in brain. In fact, the concentration of L-glutamate binding sites in cortical synaptic plasma membranes was significantly increased in the 20-day-old offspring of ethanol-fed rats. In contrast to the cortex, binding to cerebellar synaptic plasma membranes was comparable in 20-day-old offspring of control and ethanol-fed rats. Despite transient alterations in the concentrations of L-glutamate binding sites in brain and synaptic plasma membranes, the affinity of the sites for L-glutamate (Kd) was consistently normal in the 14- to 26-day-old offspring of ethanol-fed rats. |
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