Effects of salvianolic acids on endothelial cells against damage induced by cholestane-3β-5α-6β-triol

Objective To investigate the effects of salvianolic acids on human umbilical vein endothelial cells(HUVEC) against damage induced by cholestane-3β-5α-6β-triol (chol-triol).Methods The viability of HUVEC was measured by MTT method. The apoptosis of HUVEC induced by chol-triol was detected by flow cytometry and TUNEL assay. The production of malondialdehyd (MDA) in HUVEC was tested by thiobarbaturic acid (TBA) assay.Results The viability of HUVEC treated with chol-triol 100μmol/L decreased by 39. 8% while salvianolic acids 100μg/ml increased by 27. 9%. The apoptotic rate of HUVEC measured by PI staining increased from 6% -8% to 17% -20% after chol-triol treatment for 12 h. Salvianolic acids100μg/ml reduced the apoptotic rate to 10% -14% after treatment HUVEC for 1 h prior to chol-triol treatment. In another experiment, chol-triol increased the number of TUNEK-positive cells 5 times, but salvianolic acids 10μg/ml and 100μg/ml reduced the number of TUNEL-positive cells by 36. 9% and 61.2%, respectively. The production of MDA in HUVEC increased by 120. 7% after chol-triol treatment for 12 h. Salvianolic acids 10 gg/ml and 100 pg/ml also decreased the concentration of MDA by 28.7% and 39. 8%, respectively.Conclusion Salvianolic acids has protective effect on endothelial cells against damage induced by chol-triol.

Effects of salvianolic acids on endothelial cells against damage induced by cholestane-3beta-5alpha-6beta-triol

OBJECTIVE: To investigate the effects of salvianolic acids on human umbilical vein endothelial cells (HUVEC) against damage induced by cholestane-3beta-5alpha-6beta-triol (chol-triol). METHODS: The viability of HUVEC was measured by MTT method. The apoptosis of HUVEC induced by chol-triol was detected by flow cytometry and TUNEL assay. The production of malondialdehyd (MDA) in HUVEC was tested by thiobarbaturic acid (TBA) assay. RESULTS: The viability of HUVEC treated with chol-triol 100 micro mol/L decreased by 39.8% while salvianolic acids 100 micro g/ml increased by 27.9%. The apoptotic rate of HUVEC measured by PI staining increased from 6% - 8% to 17% - 20% after chol-triol treatment for 12 h. Salvianolic acids 100 micro g/ml reduced the apoptotic rate to 10% - 14% after treatment HUVEC for 1 h prior to chol-triol treatment. In another experiment, chol-triol increased the number of TUNEL-positive cells 5 times, but salvianolic acids 10 micro g/ml and 100 micro g/ml reduced the number of TUNEL-positive cells by 36.9% and 61.2%, respectively. The production of MDA in HUVEC increased by 120.7% after chol-triol treatment for 12 h. Salvianolic acids 10 micro g/ml and 100 micro g/ml also decreased the concentration of MDA by 28.7% and 39.8%, respectively. CONCLUSION: Salvianolic acids has protective effect on endothelial cells against damage induced by chol-triol.