Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells |
| |
Authors: | Wan Hong An Yihua Zhang Zeshun Zhang Yazhuo Wang Zhongcheng |
| |
Institution: | Beijing Neurosurgical Institute, Beijing 100050, China |
| |
Abstract: | OBJECTIVE: To explore the factors which induce differentiation of embryonic neural stem cells. METHODS: Rat embryonic neural stem cells were co-cultured with newborn rat Schwann cells in serum-free medium. The phenotype and specific-markers including tubulin-beta, glial fibrillary acidic protein (GFAP) and galactorcerebroside (GalC), were demonstrated by phase contrast microscopy and double immunofluorescence staining. RESULTS: Overall, 80% +/- 5% of neural stem cells protruded several elongated processes and expressed tubulin-beta antigen at high levels, while 20 +/- 3% of them protruded several short processes and were GalC or GFAP positive. CONCLUSION: The factors secreted by Schwann cells could induce rat embryonic neural stem cell to differentiate. |
| |
Keywords: | Schwann cells stem cells cell differentiation |
本文献已被 CNKI 万方数据 PubMed 等数据库收录! |
| 点击此处可从《中华医学杂志(英文版)》浏览原始摘要信息 |
| 点击此处可从《中华医学杂志(英文版)》下载免费的PDF全文 |