Altered expression of NDST-1 messenger RNA in puromycin aminonucleoside nephrosis |
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| Authors: | Nakayama Kenji Natori Yumiko Sato Toshinobu Kimura Tomoyoshi Sugiura Akira Sato Hiroshi Saito Takao Ito Sadayoshi Natori Yasuhiro |
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| Affiliation: | Research Institute, International Medical Center of Japan, Tokyo, Japan. |
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| Abstract: | |
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| Keywords: | cDNA, complementary DNA dCTP, deoxycytidine triphosphate DMEM, Dulbecco's modified Eagle medium EDTA, ethylenediaminetetraacetate FCS, fetal calf serum GAG, glycosaminoglycan GAPDH, glyceraldehyde-3-phosphate dehydrogenase GBM, glomerular basement membrane GEC, glomerular epithelial cell GMC, glomerular mesangial cell GlcN, unsubstituted glucosamine GlcNAc, N-acetylglucosamine HEPES, N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid HSPG, heparan sulfate proteoglycan kb, kilobase mRNA, messenger RNA NDST, heparan sulfate GlcNAc N-deacethylase/PAPS:GlcN N-sulfotransferase PAN, puromycin aminonucleoside PAPS, adenosine 3′-phosphate 5′-phosphosulfate PAS, periodic acid-Schiff PBS, phosphate-buffered saline solution PCR, polymerase chain reaction ROS, reactive oxygen species SDS, sodium dodecyl sulfate SSC, standard saline citrate |
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