大鼠局灶性脑缺血再灌注后脑组织肾上腺髓质素的表达

目的 探讨肾上腺髓质素（adrenomedullin，ADM）在不同月龄大鼠脑缺血再灌注（I／R）脑组织的表达情况。方法 采用栓线法制成大鼠大脑中动脉I／R模型，阻断血流2h后再灌注4h。应用免疫组织化学染色法检测不同月龄段大鼠局灶性脑I／R后血浆ADM。结果 正常大鼠脑内即有ADM表达，假手术后ADM表达略有增加，但与正常对照组相比无明显差异（P〉0．05）；大鼠脑I／R后ADM免疫阳性细胞增多，与正常对照组及假手术组相比差异显著（均P〈0．05）；大鼠脑I/R后缺血侧及缺血对侧ADM免疫阳性细胞均增多，但以缺血侧区域增多最为明显（P〈0．05）。老龄大鼠脑I／R后ADM免疫阳性细胞数明显高于青年大鼠（P〈0．05）。结论 脑I／R后ADM表达增强，ADM表达增强与血管硬化相关。

Expression of cerebral adrenomedullin in rats with acute focal cerebral infarction and reperfusion

Objective To investigate the expressions of adrenomedullin(ADM) in rats aged different months with acute focal cerebral infarction and reperfusion(I/R).Methods The model of middle cerebral artery occlusion in rat was induced by the intraluminal suture occlusion,ischemia for 2 hours then reperfusion.The expression of ADM after focal cerebral I/R was measured by immunohistochemistry.Results There were ADM positive immunocytes in the brains of control group,but slightly increased in sham group(P>0.05).ADM positive immunocytes increased significantly after focal cerebral I/R,comparing to that in sham group and control group(P<0.05).ADM positive immunocytes increased both in the ischemia area and non-ischemia area,especially in the ischemia area(P<0.05).ADM positive immunocytes increased in elderly rats than that in the young(P<0.05).Conclusions The significantly increased expression of ADM after focal cerebral I/R should be related to degree of vascular sclerosis.