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| 人脐带沃顿胶间充质干细胞对重型再生障碍性贫血调节性T细胞及Foxp3基因的影响 | |
| 引用本文： | 郭鹏,刘传方,赵雯.人脐带沃顿胶间充质干细胞对重型再生障碍性贫血调节性T细胞及Foxp3基因的影响[J].山东大学学报(医学版),2011,49(9):71-76. |
| 作者姓名： | 郭鹏 刘传方 赵雯 |
| 作者单位： | 1. 山东大学西区校医院内科,济南,250012 2. 山东大学齐鲁医院血液科,济南,250012 3. 山东省荣军医院耳鼻喉科,济南,250013 |
| 摘 要： | 目的研究脐带沃顿胶间充质干细胞(WJCs)对重型再生障碍性贫血(SAA)患者外周血CD4+CD25+调节性T细胞及Foxp3基因表达水平的影响,探讨WJCs对SAA患者T淋巴细胞(TLCs)免疫调节作用的可能机制。方法从人脐带中分离培养WJCs,通过流式细胞术检测其表面标记并进行鉴定;采用密度梯度离心法从SAA患者外周血中分离TLCs;在植物凝集素(PHA)刺激下,将SAA患者TLCs(1×105个)进行体外培养,实验组加入不同数量级WJCs(1×103、1×104、1×105个)共培养,3 d后采用MTT法检测淋巴细胞抑制率;流式细胞仪检测实验组及正常对照组SAA患者TLCs中CD4+CD25+T细胞比例的变化,RT-PCR方法检测Foxp3基因mRNA水平的变化。结果 WJCs能明显抑制SAA患者TLCs增殖,且抑制作用与WJCs呈剂量依赖性;实验组CD4+CD25+T细胞比例以及Foxp3基因表达水平均比对照组明显增加,且与TLCs增殖水平呈负相关。结论 WJCs对SAA患者TLCs的抑制作用呈剂量依赖性,通过上调Foxp3的表达而发挥CD4+CD25+调节性T细胞的免疫调节作用可能是其机制之一。 |
| 关 键 词： | 沃顿胶间充质干细胞 CD4+CD25+调节性T细胞 Foxp3基因 再生障碍性贫血 |
Effects of Wharton's jelly-derived mesenchymal stem cells from the human umbilical cord on regulatory T cells and the Foxp3 gene in severe aplastic anemia patients |
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| GUO Peng,LIU Chuan-fang,ZHAO Wen.Effects of Wharton's jelly-derived mesenchymal stem cells from the human umbilical cord on regulatory T cells and the Foxp3 gene in severe aplastic anemia patients[J].Journal of Shandong University:Health Sciences,2011,49(9):71-76. | |
| Authors: | GUO Peng LIU Chuan-fang ZHAO Wen |
| Institution: | GUO Peng1,LIU Chuan-fang2,ZHAO Wen3(1.Department of Internal Medicine,School Hospital,Shandong University,Jinan 250012,China,2.Department of Hematology,Qilu Hospital of Shandong University,3.Department of Otorhinolaryngology,Shandong Rongjun Hospital,Jinan 250013,China) |
| Abstract: | Objective To investigate effects of Wharton's jelly-derived mesenchymal stem cells(WJCs) from the human umbilical cord on CD4+CD25+ regulatory T cells and the Foxp3 gene,and their immunoregulatory mechanisms in severe aplastic anemia(SAA) patients.Methods WJCs were isolated from the human umbilical cord,expanded,and identified by morphology of culture cells and flow cytometry of stem cell markers.Using density gradient centrifugation,T-lymphocytes(TLCs) were isolated from human peripheral blood from patient... |
| Keywords: | Wharton's jelly-derived mesenchymal stem cells CD4 CD25 regulatory T cells Foxp3 gene Aplastic anemia |
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