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| 人乳头瘤病毒16型E7蛋白单克隆抗体的研制 | |
| 引用本文： | 阴秀丽,李坤,宁涛,徐昌青,陈自平. 人乳头瘤病毒16型E7蛋白单克隆抗体的研制[J]. 山东大学学报(医学版), 2011, 49(12): 62-65 |
| 作者姓名： | 阴秀丽 李坤 宁涛 徐昌青 陈自平 |
| 作者单位： | 1. 山东大学医学院,济南250012;山东省荣军总医院消化内科,济南250013 2. 山东大学附属千佛山医院消化内科,济南,250014 3. 北京市肿瘤防治研究所遗传室,北京,100142 |
| 基金项目： | 山东省科技攻关计划资助项目(2006GG3202017) |
| 摘 要： | 目的 为研究HPV16 E7蛋白在肿瘤早期预报中的作用及研制治疗性疫苗,特制备HPV16 E7的单克隆抗体.方法 将构建成的PGEX4T-1-HPV16 E7的质粒转化BL21细菌,在IPTG诱导下,稳定表达GST-HPV16E7融合蛋白,免疫Balb/c小鼠,收集免疫小鼠的脾细胞与SP2/0细胞融合,用ELISA双筛,直至检测所有孔均为E7阳性而GST阴性为止,扩大培养、建株、冻存并制备腹水、单克隆抗体.结果 成功制备出HPV16 E7的单克隆抗体,可在多种肿瘤组织细胞核中染色.结论 该抗体是HPV16 E7蛋白特异性的McAb. |
| 关 键 词： | 消化道肿瘤 人乳头瘤病毒16E7 单克隆抗体 |
Preparation of a monoclonal antibody against the human papillomavirus type 16 E7(HPV 16 E7) protein |
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| YIN Xiu-li , LI Kun , NING Tao , XU Chang-qing , CHEN Zi-ping. Preparation of a monoclonal antibody against the human papillomavirus type 16 E7(HPV 16 E7) protein[J]. Journal of Shandong University:Health Sciences, 2011, 49(12): 62-65 | |
| Authors: | YIN Xiu-li LI Kun NING Tao XU Chang-qing CHEN Zi-ping |
| Affiliation: | YIN Xiu-li1,2,LI Kun3,NING Tao4,XU Chang-qing3,CHEN Zi-ping3 (1.Medical School,Shandong University,Jinan 250012,China,2.Department of Gastroenterology,Rongjun Hospital of Shandong Province,Jinan 250013,3.Department of Gastroenterology,Qianfoshan Hospital Affliated to Shandong University,Jinan 250014,4.Genetics,Beijing Institute for Caneer Research,Beijing 100142,China) |
| Abstract: | Objective An HPV 16 E7 monoclonal antibody was prepared.This antibody will be useful for diagnosing early cancers and developing therapeutic vaccines.Method A plasmid constructed with PGEX4T-1-HPV16 E7 was transformed into E.coli BL21,and expression of the fusion protein was induced by IPTG.Spleen cells from immunized Balb/c mice were fused with SP2/0 cells by a routine method,and the valence of the antibody was evaluated using ELISA.Expansion,isolation and freezing of clones were carried out,and finally an... |
| Keywords: | Digestive tract tumors Human papillomavirus 16 E7 Monoclonal antibody |
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