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| miR-122表达载体的构建及其对HepG2.2.15细胞增殖的抑制作用 | |
| 引用本文： | 范春光,王春梅,王燕,李丽,孙汶生,刘玉刚,李瑞峰.miR-122表达载体的构建及其对HepG2.2.15细胞增殖的抑制作用[J].山东大学学报(医学版),2011,49(10):118-121,126. |
| 作者姓名： | 范春光 王春梅 王燕 李丽 孙汶生 刘玉刚 李瑞峰 |
| 作者单位： | 1. 山东大学医学院病理生理学研究所,济南,250012 2. 山东大学医学院微生物研究所,济南,250012 3. 山东大学医学院免疫研究所,济南,250012 |
| 基金项目： | 国家自然科学基金青年基金资助项目(No.30801036) |
| 摘 要： | 目的构建miR-122表达载体并检测其表达对HepG2.2.15细胞恶性表型的逆转作用。方法以HepG2.2.15细胞基因组DNA为模板,PCR扩增miR-122前体cDNA序列,以质粒pSilencer3.1-H1 neo为母本,构建miR-122表达载体将其转染HepG2.2.15细胞。表达后,利用ELISA检测HepG2.2.15细胞HBV复制和表达的变化,利用CCK8检测细胞增殖的变化。结果构建的miR-122表达载体可在HepG2.2.15细胞中表达。转染后HepG2.2.15细胞中HBV复制、表达以及细胞增殖均被抑制。结论 miR-122表达载体可在HepG2.2.15细胞中有效表达,其表达可抑制HBV复制、表达以及细胞增殖。 |
| 关 键 词： | miR-122 表达载体 肝炎病毒 乙型 癌 肝细胞 HepG2.2.15 |
Construction of an miR-122 expression vector and its inhibitory effects on proliferation of HepG2.2.15 cells |
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| FAN Chun-guang,WANG Chun-mei,WANG Yah,LI Li,SUN Wen-sheng,LIU Yu-gang,LI Rui-feng.Construction of an miR-122 expression vector and its inhibitory effects on proliferation of HepG2.2.15 cells[J].Journal of Shandong University:Health Sciences,2011,49(10):118-121,126. | |
| Authors: | FAN Chun-guang WANG Chun-mei WANG Yah LI Li SUN Wen-sheng LIU Yu-gang LI Rui-feng |
| Institution: | FAN Chun-guang~1,WANG Chun-mei~2,WANG Yan~1,LI Li~1,SUN Wen-sheng~3,LIU Yu-gang~1,LI Rui-feng~1 (1.Department of Pathophysiology,2.Department of Microbiology,3.Institute of Immunology,School of Medicine,Shandong University,Jinan 250012,China) |
| Abstract: | Objective To construct an miR-122 expression vector and examine the effect of miR-122 expression in reversing the malignant phenotype of the HepG2.2.15 cell line.Methods Genomic DNA of the HepG2.2.15 cell line was used as a template and the target gene fragment was PCR-amplified.The product was confirmed by enzymatic digestion and sequencing.The vector was transfected into HepG2.2.15 cells.HBsAg and HBeAg in the supernatant from cell cultures were measured,and HBV-DNA level was detected by real-time PCR.Cel... |
| Keywords: | miR-122 Expression vector Hepatitis B virus Carcinoma hepatocellular HepG2 2 15 |
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