| p16基因对脑胶质瘤细胞作用的实验研究 |
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| 引用本文： | 陈一招,徐如祥,邹琳. p16基因对脑胶质瘤细胞作用的实验研究[J]. 肿瘤, 2000, 20(4): 252-254 |
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| 作者姓名： | 陈一招 徐如祥 邹琳 |
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| 作者单位： | 第一军医大学珠江医院神经外科!广州510282 |
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| 摘 要： | 目的 探索ｐ１６基因在脑胶质瘤发生发展过程中的作用及ｐ１６基因在脑胶质瘤临床诊断、治疗中的应用前景。方法 采用脂质体转染的方法，将外源野生型ｐ１６基因导入胶质瘤细胞株Ｕ２５１、Ｃ６，筛选阳怀克隆。同时以空载体质粒ｐＣＤＮＡ３为对照，免疫组化检测ｐ１６基因表达，用ＭＴＴ 测定瘤细胞生长曲线及对转染的瘤细胞在裸鼠体内形成瘤块的变化进行分析。结果 转染ｐ１６基因的Ｕ２５１、Ｃ６细胞有外源ｐ１６基因的整合
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| 关 键 词： | 脑胶质瘤 p16基因 诊断 治疗 实验研究 |
EXPERIMENTAL RESEARCH ON THE EFFECTS OF p16 GENE IN HUMAN GLIOMA CELL LINES |
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| CHEN Yi zhao,XU Ru xiang,ZOU Lin. Neurosurgery Department,Zhujiang Hospital,First Military Medical University,Huangzhou, China. EXPERIMENTAL RESEARCH ON THE EFFECTS OF p16 GENE IN HUMAN GLIOMA CELL LINES[J]. Tumor, 2000, 20(4): 252-254 |
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| Authors: | CHEN Yi zhao XU Ru xiang ZOU Lin. Neurosurgery Department Zhujiang Hospital First Military Medical University Huangzhou China |
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| Affiliation: | CHEN Yi zhao,XU Ru xiang,ZOU Lin. Neurosurgery Department,Zhujiang Hospital,First Military Medical University,Huangzhou,510282 China |
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| Abstract: | Objective To determine the effects of p16 gene on human glioma cell lines. Methods p16 gene was transfected into U251 and C6 glioma cell lines by lipofectin. Integration and expression of exogenous p16 gene were detected. MTT colorimetric assay was used to measure cell growth. Tumor mass formation of glioma cells was observed in nude mice. Results Expression of p16 gene was demonstrated by immunohistochemistry. The growth rate of both p16 gene transfected U251 and C6 reduced Tumor mass formation of tumor cells in nude mice was inhibited by transfected p16 gene. Conclusion The expression of p16 gene can inhibit the growth of glioma and tumor formation in nude mice. $$$$ |
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| Keywords: | Glioma Gene p16 Transfection |
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