| 偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学分析 |
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| 引用本文： | 胡建军,陈泽奇,钟广伟,李炜,尹耀慧. 偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学分析[J]. 中国中医药信息杂志, 2008, 15(8): 34-37 |
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| 作者姓名： | 胡建军 陈泽奇 钟广伟 李炜 尹耀慧 |
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| 作者单位： | 中南大学湘雅医院中西医结合研究所,湖南长沙,410008 |
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| 基金项目： | 国家自然科学基金青年基金，湖南省重点学科资助项目 |
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| 摘 要： | 目的筛选与偏头痛肝阳上亢型相关的蛋白质，进一步揭示偏头痛肝阳上亢证的分子机制。方法用电刺激SD大鼠三叉神经节，并灌服附子汤的方法制造偏头痛肝阳上亢型模型。采用双向凝胶电泳（2-DE）技术分离偏头痛肝阳上亢型大鼠脾脏淋巴细胞的总蛋白，PDQuest7．0图像分析软件识别差异表达的蛋白质点，基质辅助激光解吸电离飞行时间质谱得到相应的肽质量指纹图，搜索数据库鉴定蛋白质。结果鉴定到的10个差异蛋白分别为：氯通道蛋白-1、硫氧还蛋白过氧化物酶-I、硫氧还蛋白过氧化物酶-Ⅱ、专利蛋白W0979348序列、Rho GDP解离抑制因子α、肌动蛋白2／3复合物、神经微丝轻肽、蛋白酶体、热休克蛋白-27、膜联蛋白-A1。结论这些蛋白可能与偏头痛肝阳上亢证的形成有关，本实验为进一步揭示偏头痛肝阳上亢证的发病机制提供了新的线索。
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| 关 键 词： | 蛋白质组学 偏头痛 肝阳上亢证 脾脏淋巴细胞 大鼠 |
Comparative Proteomics Analysis on Splenic Lymphocytes in Migraine Rat Model with Hyperactivity of Liver-yang |
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| HU Jian-jun,CHEN Ze-qi,ZHONG Guang-wei,et al. Comparative Proteomics Analysis on Splenic Lymphocytes in Migraine Rat Model with Hyperactivity of Liver-yang[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2008, 15(8): 34-37 |
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| Authors: | HU Jian-jun CHEN Ze-qi ZHONG Guang-wei et al |
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| Affiliation: | HU Jian-jun,CHEN Ze-qi,ZHONG Guang-wei,et al (Xiangya Hospital Affiliated to Central South University,Changsha 410008,China) |
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| Abstract: | Objective To explore the molecule mechanisms of migraine with hyperactivity of liver-yang related proteins. Methods Animal model of migraine with hyperactivity of liver-yang was established through electrical trigeminal ganglion stimulation,and by administering orally with Fuzi decoction. The total proteins of splenic lymphocytes in the rats were separated by immobilized pH gradient-based two-dimensional gel electrophoresis. With coomassie blue staining,the 2-DE images were analyzed by PDQuest 7.0 software.... |
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| Keywords: | proteomics migraine hyperactivity of liver-yang splenic lymphocytes rat |
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