当归、川芎、红花不同组合方式提取物的指纹图谱比较

 目的以指纹图谱的方式,研究当归(A)、川芎(B)、红花(C)不同组合方式提取物中化学成分的差异。方法将方药组合成5组(ABC、AB+C、AC+B、BC+A、A+B+C)进行提取,并用RP-HPLC法进行分析,比较其色谱指纹图谱。结果5种组合提取液中化学成分组成存在差异,ABC合煎提取物中主要化学成分的提取率大于其他各组的提取物,且5种组合的化学成分组成比例的差异较大。结论当归、川芎、红花不同组合提取物中主要化学成分组成及其共有成分比例存在差异,3味药合煎成分煎出较完全。

Fingerprint Comparison of Decoction Composed by Different Ratio of Radix Angelicae Sinensis, Rhizoma Chuanxiong and Flos Carthami

OBJECTIVE To study the difference of chemical composition among the extracts of radix angelicae sinensis,rhizoma chuanxiong and flos carthami with fingerprint technology.METHODS The ingredients were divided into 5 groups(ABC,AB C,AC B,BC A,A B C) and extracted by normal methods.The samples were determined by RP-HPLC and the fingerprints were compared.RESULTS The ratio of the compostions and their main chemical substances were the different among the five extracts,and the extract rate of the main chemical substances were higher in decoction A B C than any other decoctions.CONCLUSION There are some chemical substances and compostion's ratio differences in the extracts of radix angelicae sinensis,rhizoma chuanxiong and flos carthami prepared by decocting,and the decoction A B C is better than any other decoctions.