BMP7 induces the differentiation of bone marrow-derived mesenchymal cells into chondrocytes |
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Authors: | Xiaodan Bai Guangze Li Can Zhao Hongzhang Duan Fujun Qu |
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Institution: | (1) Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, 148 Baojian Road, Harbin, 150086, Heilongjiang Province, China; |
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Abstract: | Injured articular cartilage has a poor capacity for spontaneous healing. So far, a satisfactory solution to repair the injured
cartilage has not been found, but transgenic therapy might be a promising treatment. This study aims to evaluate the potential
of transfecting bone morphogenetic protein-7 (BMP-7), a secretory protein, into bone marrow-derived mesenchymal stem cells
(BMSCs), in inducing the differentiation of bone marrow stromal cells into chondrocytes in vitro. The phenotypes of the cells
were observed by alcian blue staining and H&E staining with an inverted microscope. The glycosaminoglycan (GAG) content of
BMSCs transfected with pcDNA3.1-BMP7 or induced by inducing medium was examined after 7, 14, or 21 days of incubation. A standard
curve as reference for BMSCs’ GAG content was plotted using galacturonic acid. The content of type II collagen in culture
medium was detected by ELISA. Our results demonstrated that BMP7-transfected BMSCs or BMSCs incubated with inducing medium
possess the ability to differentiate into chondrocytes. BMP7-induced BMSCs secrete type II collagen and GAG. There was no
significant difference between BMP7-induced BMSCs in their secreted protein content when compared with the positive control
group (TGF-β1 and dexamethasone) (P > 0.05), but there was significant difference in the secreted protein profile when compared with the negative control group
(P < 0.05). |
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