| Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca~(2 ) |
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| 引用本文： | 周舟,王小华,Igisu Hideki,林远,楼淑芬,Matsuoka Masato,程天民,余争平. Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca~(2 )[J]. 中国人民解放军军医大学学报, 2002, 17(3) |
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| 作者姓名： | 周舟 王小华 Igisu Hideki 林远 楼淑芬 Matsuoka Masato 程天民 余争平 |
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| 作者单位： | Institute of Combined Injury，Third Military Medical University，Chongqing 400038，China，Institute of Combined Injury，Third Military Medical University，Chongqing 400038，China，Department of Environmental Toxicology，University of Occupational and Environmental Health，Kitakyushu 807-85555，Japan，Institute of Combined Injury，Third Military Medical University，Chongqing 400038，China，Department of Environmental Toxicology，University of Occupational and Environmental Health，Kitakyushu 807-85555，Japan，Department of Environmental Toxicology，University of Occupational and Environmental Health，Kitakyushu 807-85555，Japan，Institute of Combined Injury，Third Military Medical University，Chongqing 400038，China，Institute of Combined Injury，Third Military Medical University，Chongqing 400038，China |
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| 基金项目： | in part by Natural Sciences Foundation of China (No. 39870239)，by the Sasagawa Fellowship,Japan. |
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| 摘 要： | Objective: To explore the effects of γ-irradiation on mitogen-activated protein kinases (MAPKs) and role of intracellular calcium in this event in intestinal epithelial cell line 6 (IEC-6 cells). Methods: After cultured rat IIEC-6 cells with or without the pretreatment of intracellular Ca2 chelator were exposed to Y-ir-radiation of 6 Gy, the total and phosphorylated MAPKs in the cells were determined with Western blotting and apoptosis was examined with flow cytometry. Activities of Extracellular signal-regulated protein kinase (ERK) and p38 MAPK were determined by using immuoprecipitation followed by Western blotting. Results: In response to γ-irradiation, phosphorylation of ERK was not significantly observed, while the levels of phosphorylated c-Jun NH2-terminal kinase (JNK) and p38 MAPK were increased in 30 min and reached the peak 2 h after exposure to 6 Gy γ-irradiation, though the cell viability was significantly lowered 12 h. On the other hand, no obvious changes were seen in the total protei
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| 关 键 词： | γ-irradiation |
Differential activation of mitogen-activated protein kinases by γ-irradiation in IEC-6 cells: Role of intracellular Ca2+ |
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| ZHOU Zhou,WANG Xiao-hua,Igisu Hideki,LIN Yuan,LOU Shu-fen,Matsuoka Masato,CHENG Tian-min,YU Zheng-ping. Differential activation of mitogen-activated protein kinases by γ-irradiation in IEC-6 cells: Role of intracellular Ca2+[J]. Journal of Medical Colleges of PLA(China), 2002, 17(3) |
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| Authors: | ZHOU Zhou WANG Xiao-hua Igisu Hideki LIN Yuan LOU Shu-fen Matsuoka Masato CHENG Tian-min YU Zheng-ping |
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| Abstract: | |
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| Keywords: | r-irradiation extracellular signal-regulated protein kinase c-Jun NH2-terminal kinase mitogen- activated protein kinases p38 MAPK intracellular Ca2 intestinal epithelial cell line 6 |
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