体外扩增的脐血细胞移植于BALB/C小鼠的实验研究

目的　探讨经体外扩增的脐血造血细胞对小鼠造血重建功能的影响 ,寻求脐血用于成人移植的可行方法。方法　将经致死剂量γ射线照射后的BALB/C小鼠 ,随机分为 4组 ,每组 10只。A组 :每只移植 2 .5× 10 6个新鲜脐血细胞 ;B组 :每只分别移植 1.2 5× 10 6个新鲜脐血细胞和 1.2 5×10 6个扩增培养 1周的脐血细胞 ;C组 :每只移植 2 .5× 10 6个扩增培养 1周的脐血细胞 ;D组 :每只仅输注 0 .5ml生理盐水。比较各组造血重建的差别。结果　在脐血细胞移植后第 13d ,A组存活 5只(5 0 % ) ,B组 8只 (80 % ) ,C组 6只 (6 0 % ) ,D组全部死亡。B组小鼠的死亡率最低。脐血细胞移植后 14d ,小鼠的WBC已开始回升 ,B组与C组的恢复明显较A组快 ,P <0 .0 5。结论　混合移植新鲜的和体外培养 1周的脐血造血细胞 ,可能是脐血移植的较好办法。

Experimental study and significance of expanded human umbilical cord blood cells in vitro transplanted to BALB/C mice

Objective To study the effect of expansion ex vivo on the hematopoietic reconstitution of umbilical cord blood (UCB) hematopoietic cells, and to find a probable way of UCB transplantation (UCBT) to be used on adults. Method Three groups of UCB hematopoietic cells were transplanted to lethally irradiated BALB/C mice: fresh cells, a half part of fresh cells and a half part of cells expanded for a week, cells expanded for a week. Results All the three groups of cells could reconstitute hematopoiesis. The cells in the latter two groups could more rapidly accelerate the recovery of early-period hematopoiesis. The death rate of the mice in second group was the lowest among three groups. Conclusion The way transplanted with a half part of fresh UCB hematopoietic cells and a half part of UCB hematopoietic cells ex vivo expanded for a week may be a probable one for adult UBCT.