| 双标记原位杂交法检测间期细胞BCR/ABL融合基因 |
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| 引用本文： | 袁长吉,王建伟,李舜华,易永林. 双标记原位杂交法检测间期细胞BCR/ABL融合基因[J]. 吉林大学学报(医学版), 2003, 29(5): 637-639 |
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| 作者姓名： | 袁长吉 王建伟 李舜华 易永林 |
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| 作者单位： | 1. 吉林大学第一医院血液肿瘤科,吉林,长春,130021
2. 吉林大学基础医学院病理解剖学教研室 |
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| 摘 要： | 目的 :应用双标记原位杂交方法检测 BCR/ ABL融合基因。方法 :BCR基因探针用地高辛标记 ,碱性磷酸酶显色 ;ABL基因用3 H- d ATP标记 ,核子乳胶放射自显影。结果 :检测 9例初治的慢性粒细胞白血病 ( CML)病人均为阳性 ,阳性细胞比例为 93% ;检测 CML来源的 K5 62细胞株阳性细胞占 98.8% ;正常人假阳性率为 0 .75 %。结论 :该方法简便、快速 ,适用于 CML微小残留病的检测
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| 关 键 词： | 白血病,髓样,慢性 原位杂交 BCR/ABL融合基因 |
| 文章编号： | 1671-587X(2003)05-0637-03 |
| 修稿时间： | 2002-08-26 |
Detection of BCR/ABL Fusion Gene in Interphase Cens with Bi-labelling Hybridization in Situ |
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| YUAN Chang ji ,WANG Jian wei ,LI Shun hua ,YI Yong lin. Detection of BCR/ABL Fusion Gene in Interphase Cens with Bi-labelling Hybridization in Situ[J]. Journal of Jilin University: Med Ed, 2003, 29(5): 637-639 |
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| Authors: | YUAN Chang ji WANG Jian wei LI Shun hua YI Yong lin |
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| Affiliation: | YUAN Chang ji 1,WANG Jian wei 2,LI Shun hua 1,YI Yong lin 1 |
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| Abstract: | Objective:To Detect BCR/ABL fusion gene with bi labelling hybridization in situ. Methods:BCR gene probe was labeled by cardiox, showed color with alkaline phosphatase. ABL gene were labeled by 3H Datp, radiating self developing with nucleon lacteprene. Results:BCR/ABL fusion gene all were positive in 9 chronic myelocytic leukemia(CML) patients in initial treatment, the ratio of positive cells was 93%, and the ratio of positive cells was 98.8% in K 562 cell strain source from CML. It showed that the results accord with objective changes. Pseudo positive rate was 0.75% in normal persons. Conclusion:The methods is both easy and fast,and it is suitable to detect minimal residual disease of chronic myelocytic leukemia (CML). [ |
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| Keywords: | Chronic myelocytic leukemia Hybridization in situ BCR/ABL fusion gene |
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