己烯雌酚对胎鼠海马神经干细胞增殖与分化的影响

目的:探讨己烯雌酚对胎鼠海马神经干细胞(neural stem cells,NSCs)的促增殖、分化作用及其机制。方法:取孕16天SD胎鼠海马NSCs培养,分别设空白对照组,己烯雌酚1×10-7 mol/L组、1×10-8 mol/L组、1×10-9 mol/L组,以及脑源性神经营养因子(BDNF)5×10-2 mg/L组;免疫荧光化学方法检测Nestin、神经元特异烯醇化酶及神经胶质酸性蛋白的表达,RT-PCR检测BDNF mRNA的含量。结果:不同剂量己烯雌酚处理各组神经球面积、积分光密度值、平均突起长度与平均突起数均增加,其中10-8 mol/L组海马NSC增殖与分化最明显,BDNF mRNA表达量亦最高(P<0.01)。结论:己烯雌酚对海马NSCs的增殖分化具有促进作用,且可能与上调BDNF的表达有关。

Influence of diethylstilbestrol on proliferation and differentiation of hippocampal neural stem cells

Objective： To investigate the mechanism of the effect of diethylstilbestrol on the proliferation and differentiation of hippocampal neural stem cells（NSCs） in fetal rats. Methods： Hippocampal neural stem cells from the embryo （gestational day 16） of fetal rats were prepared and divided into five groups： control, diethylstilbestrol 1× 10^-7 mol/L, diethylstilbestrol 1 × 10^-8 mol/L, diethylstilbestrol 1 × 10^-9 mol/L and BDNF 5 ×10^-2 mg/L. The expressions of Nestin, neurone specific enolase（NSE） and glial fibrillary acidic protein（GFAP） were determined by immunohistochemistry, and the level of brain-derived neurotrophic factor（BDNF） mRNA was detected by RT-PCR. Results： Different doses of diethylstilbestrol could increase the area of neural spheres, quantity of NSE,and GFAP-positive cells. At a concentration of 10^-8 mol/L, diethylstilbestrol facilitated the proliferation and differentiation of hippocampal NSCs of fetal rats the most, and the level of BDNA mRNA was the highest （ P 〈 0.01 ）. Conclusions ： Diethylstilbestrol enhances the proliferation of hippocampal NSCs in fetal rats,which is probably related to the up-regulation of the BDNF mRNAs＇ expression.