葡萄糖-6-磷酸脱氢酶活性浓度检测法的初步研究

目的建立一种简单、快速、可自动检测葡萄糖-6-磷酸脱氢酶（G-6-PD）在血红蛋白（Hb）中活性的方法。方法用自配的检测试剂，在全自动生化分析仪上检测抗凝血标本的G-6-PD活性，同时检测Hb浓度，并计算出G-6-PD在Hb中的活性浓度（U／gHb），对同一标本用简易快速高铁血红蛋白还原试验、G-6-P／6-GP比值法进行对比试验。结果G-6-PD活性浓度的参考范围为≥5．0U／gHb，G-6-PD活性浓度检测法与G-6-P／6-GP比值法及简易快速高铁血红蛋白还原试验检测法的检测结果具有高度一致性。G-6-PD活性浓度检测法可用不洗涤红细胞，其检测试剂及检测方法具有良好的重复性。制备溶血液时吸样量从7～13μl的G-6-PD活性浓度检测结果差异无统计学意义（P〉0．05）。直接用酸性枸橼酸右旋糖（ACD）抗凝全血比用压积红细胞检测G-6-PD活性浓度的结果高。结论G-6-PD活性浓度检测法是一种简单、快速、重复性好、操作简便的检测方法。

Preliminary study on analytical method of glucose 6 phosphate dehydrogenase active concentration

Objective To establish a simple,quick and automated method to detect the glucose-6-phosphate dehydrogenase（G-6-PD） activity. Methods To prepare the reagents of the G-6-PD activity and detect by Hitachi- 7060 automatic biochemistry analyzer,at the same time,detect the Hb concentration and then calculate the numercal value of G-6-PD active concentration（U/g Hb）, conduct the contrastive test by G-6-P/6-GP relative value method and easy methemoglobin reduction test. Results The limit of the G-6-PD active concentration was≥5.0 U/g Hb. There was good consistensy among the results of G-6-PD active concentration analytical method,G-6-P/6-GP relative value method and easy methemoglobin reduction test. Unwashed red blood cells could be used to detect the G-6-PD active concentration. There was satisfactory reproducibility of the reagents and the analytical method of G-6-PD active concentration. There was no significant difference of the G-6-PD active concentration in the application of sample volume from 7 μl to 13μl in procedure of hemolysate preparation. There was higher G-6-PD active concentration detected by whole blood anticoagulated with ACD than that by packed red cells（P〈0.05）. Conclusion The analytical method of G-6-PD active concentration is a simple,quick and automated method with satisfactory reproducibility for detecting G-6-PD activity.