Association of uromodulin gene polymorphism with diabetic kidney disease in Han population in Tianjin of China

Objective To investigate the association of single nucleotide polymorphism (SNP) rs13333226 in uromodulin (UMOD) gene with diabetic kidney diseases (DKD) in Han population in Tianjin, China. Methods A total of 210 type 2 diabetes (T2DM), 90 normal controls (NC) and 280 DKD patients were recruited. According to the level of estimated glomerular filtration rate (eGFR), the DKD subjects were further subdivided into three groups: GFR≥90 ml/min group (n=105), 60 ml/mim≤GFR＜90 ml/min group (n=84) and GFR＜60 ml/min group (n=91). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for UMOD rs13333226C genotyping. Results The frequencies of AA, GA, GG genotype were 27.8%, 58.9%, 13.3% in NC group and 41.0%, 48.6%, 10.5% in T2DM group and 54.3%, 36.1%, 9.6% in DKD group．The frequency of G allele was 42.8% in NC group, 34.8% in T2DM group and 27.7% in DKD group. The genotype distribution of UMOD was statistically significant between NC group and DKD group, and between T2DM group and DKD group (P＜0.05). G allele of UMOD was an independent protective gene polymorphism of DKD in Logistic regression (B=-0.248, Wald=8.012, P=0.021, OR=0.780, 95%CI 0.612-0.968). Conclusion The G allele of UMOD gene may be an independent protective factor of DKD in Han population in Tianjin, China.