血浆miR-451水平与子宫内膜异位症的关系

目的：探讨血浆微小RNA451（miR-451）与子宫内膜异位症（EMs）的关系，并观察miR-451对体外培养的子宫内膜基质细胞的增殖和迁移的作用。方法：选取2014年1月—2015年5月于深圳市罗湖区人民医院就诊的EMs患者（EMs组）54例，正常对照组59例，采血并提取血浆总RNA。采用实时荧光定量聚合酶链反应（real-time qPCR）检测血浆miR-451的表达强度。通过CCK-8实验检测miR-451对异位子宫内膜基质细胞增殖的调控作用；采用损伤愈合实验探讨过表达miR-451后异位子宫内膜基质细胞的迁移变化。结果：EMs患者血浆miR-451表达水平低于对照组[（0.53±0.14） vs. （1.00±0.20），t=14.50，P＜0.01]；过表达miR-451后EMs基质细胞的相对迁移活性下降[（1.00±0.12） vs. （0.74±0.02），t=4.73，P=0.001]。结论：EMs患者血浆miR-451水平减低；miR-451可能通过抑制异位内膜基质细胞增殖和迁移发挥作用。

The Relationship between Plasma miR-451 and Endometriosis

Objective: To analyze the level of plasma miR-451 in patients with endometriosis (EMs), and to investigate the effect of miR-451 on the proliferation and migration of the stromal cells cultured in vitro. Methods：We recruited 54 cases of EMs and 59 healthy volunteers from January 2014 to May 2015 at People′s Hospital of Luohu District. Total RNA from plasma were extracted, and the expression of plasma miR-451 was analyzed with real-time qPCR. The proliferation viability of stromal cells was detected by CCK-8 assay, and migration by the wound-healing assay. Results：The level of plasma miR-451 in EMs patients was significantly lower than that in healthy volunteers [(0.53±0.14) vs. (1.00±0.20), t=14.50, P＜0.01]. Overexpression of miR-451 in the in vitro cultured stromal cells significantly inhibited the proliferation and migration of stromal cells[(1.00±0.12) vs. (0.74±0.02), t=4.73, P=0.001]. Conclusions：The level of plasma miR-451 is lowered in patients with EMs. miR-451 may participate in the pathophysiological mechanism of EMs by the inhibition of proliferation and migration of ectopic stromal cells.