人卵泡液蛋白质组学的研究进展

卵泡液是由卵母细胞、颗粒细胞和膜细胞等共同分泌的一种含多种蛋白质的生物液，其作为卵母细胞生长的微环境或培养基，直接影响到卵泡的发育和卵母细胞的成熟。过去十几年，对卵泡液蛋白质组学的研究主要集中于新蛋白筛选及疾病相关蛋白等的识别上。本文从卵泡液新蛋白的筛选、年龄相关生物学标志鉴定、确定卵泡成熟标志、疾病相关标志的筛选和体外受精（IVF）成功的标志等5方面介绍近年卵泡液蛋白质组学的相关研究。筛选和鉴定某些生殖和发育相关的关键蛋白，并可能成为卵泡液中的生物标志，可用于预测卵母细胞的质量、受精及胚胎发育潜能。

Research Progress of Human Follicular Fluid Proteomics

Follicular fluid is a biological fluid containing a varity of the proteins and factors secreted by oocytes, granulosa cells and theca cells, which is important for the follicular development and oocyte maturation as the medium or micro-environment of oocyte growth. Over the past ten years, the proteomic studies of follicular fluid were focused on the identification of some novel proteins and screening of disease-related proteins. In this article, five aspects related to the proteomic studies of follicular fluid were reviewed as follows: some new proteins identified from follicular fluid, the age-related biomarkers, some definite biomarkers of follicular maturation, the disease-related markers, and the biomarkers related to IVF success. It is possible that the quality of follicle and oocyte, and the potential of fertilization and embryo development, be predicted by some potential biomarkers of follicular fluid that are the key reproduction-related and（or） developemnt-related proteins screened and identified from those proteomic studies.