抗氧化剂对染尘大鼠血液中抗氧化酶活性的影响

目的:探讨抗氧化剂茶多酚(TP)联合维生素C(VitC)、维生素E(VitE)对染尘大鼠血液中抗氧化酶活性的影响.方法:40只雄性Wistar大鼠随机分为5组:对照组、石英粉尘组(SiO2组)、TP组、TP VitC组和TP VitE组,每组均为8只.分别检测各组大鼠血液中一氧化氮(NO)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)的含量.结果:TP联用VitC、VitE可明显降低染尘大鼠血液中NO、MDA及NOS的活性及显著提高染尘大鼠血液中SOD、GSH-Px的活性(与SiO2组比较均有显著差异:P<0.05).结论:茶多酚联合维生素C、维生素E对石英粉尘引起的氧化损伤具有较好的抑制作用.

EFFECTS OF ANTIOXIDANT ON ANTIOXIDASE ACTIVITY IN BLOOD OF SILICOTIC RATS

Objective:To investigate the effects of tea polyphenols(TP) combined with vitamin C and vitamin E on antioxidase activity in blood of silicotic rats.Methods:40 male Wistar rats were used and randomly divided into five groups: control group,silica(SiO_2) group,TP group,TP+VitC group and TP+VitE group.Each group had 8 rats.The activity of NO,MDA,NOS,SOD and GSH-Px in blood were detected respectivly.Results:TP combined with vitamin C,vitamin E could decrease the NO,MDA,NOS activity and increase the SOD,GSH-Px activity in blood of silicotic rats significantly(compared with SiO_2 group:<0.05).Conclusions:TP combined with Vit C,Vit E has certain inhibitory effects on oxidative damages induced by silica dust.