肺癌患者外周血调节性T细胞和IL-10的检测及其意义

  目的  探讨肺癌患者外周血调节性T细胞和IL-10检测的诊断意义及与临床特征相关性。  方法  采用流式细胞仪检测60例肺癌患者外周血中CD4+CD25+T细胞的表达水平, 同时采用ELISA法检测IL-10的表达水平。并随机选30例健康体检者作为对照组。  结果  肺癌患者外周血中CD4+CD25+T细胞和IL-10的表达水平明显高于健康对照组（P < 0. 05）; CD4+CD25+T细胞与IL-10的检测结果呈正相关; 肺癌患者外周血中CD4+CD25+T细胞和IL-10的表达水平与临床分期有关（P < 0. 05）, 与病理类型无关（P>0. 05）。  结论  检测肺癌患者外周血中CD4+CD25+T细胞和IL-10的表达水平, 有助于肺癌患者病情的评估和治疗。 

Detection of CD4+CD25+ T-Cells and IL-10 in Peripheral Blood of Lung Cancer Patients ant Its Significance

  Objective  This study discusses the relationship of the CD4+CD25+T-cells and IL-10 in the peripheral blood of lung cancer patients with clinicopathologic features.  Methods  The amounts of CD4+CD25+T-cells in the peripheral blood samples from 60 patients were measured using flow cytometry, whereas the amounts of IL-10 were measured using enzyme-linked immunosorbent assay. Blood samples from 30 healthy volunteers were also collected and examined.  Results  The amounts of CD4+CD25+T-cells and IL-10 in the peripheral blood samples from lung cancer patients were significantly higher than those from the healthy volunteers (P < 0.05). Moreover, the amounts of CD4+CD25+T-cells and IL-10 were positively correlated. Finally, the amounts of CD4+CD25+T-cells and IL-10 were correlated with the clinical stage of the disease (P < 0.05) but were not associated with the pathological classification (P > 0.05).  Conclusion  Determining the amounts of CD4+CD25+ T-cells and IL-10 may help in the assessment and therapy of lung cancer patients.