| 分光光度法测定桑元止渴胶囊中多糖含量 |
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| 引用本文： | 黄雪峰,刘志刚,罗新根,吴新荣. 分光光度法测定桑元止渴胶囊中多糖含量[J]. 解放军药学学报, 2012, 28(4): 334-335 |
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| 作者姓名： | 黄雪峰 刘志刚 罗新根 吴新荣 |
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| 作者单位： | 广州军区广州总医院药剂科,广东广州,510010 |
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| 基金项目： | 广东省科技计划项目,No.2008A030201031 |
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| 摘 要： | 目的建立分光光度法测定桑元止渴胶囊中多糖含量的方法。方法采用分光光度法,以无水葡萄糖为对照,桑元止渴胶囊样品和对照品在苯酚、硫酸作用下显色,室温放置30 min,波长485 nm处测定吸光度。结果多糖浓度在13.65～95.55 mg·L-1范围内,吸光度与其浓度呈线性关系,回归方程A=0.0112 C+0.0231,r=0.9994。结论分光光度法可以用于桑元止渴胶囊多糖的测定。
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| 关 键 词： | 桑元止渴胶囊 多糖 分光光度法 |
Determination of Polysaccharides in Sangyuan Zhike Capsule by Spectrophotometric Method |
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| HUANG Xue-feng , LIU Zhi-gang , LUO Xin-geng , WU Xin-rong. Determination of Polysaccharides in Sangyuan Zhike Capsule by Spectrophotometric Method[J]. Pharmaceutical Journal of Chinese People's Liberation Army, 2012, 28(4): 334-335 |
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| Authors: | HUANG Xue-feng LIU Zhi-gang LUO Xin-geng WU Xin-rong |
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| Affiliation: | Department of Pharmacy,General Hospital of Guangzhou Military Command,Guangzhou 510010,China |
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| Abstract: | Objective To establish a method of determination of polysaccharides in Sangyuan Zhike capsule by spectrophotometric method.Methods Spectrophotometry was used with anhydrous glucose as reference substance.Sangyuan Zhike capsule and reference substance were treated under the action of phenol-sulphuric acid at room temperature for 30 min,and absorbance(A) was determined at a wavelength of 485 nm.Results The linear relationship was good when the sugar concentration ranged from 13.65 to 95.55 mg · L-1.The regression equation A=0.0112C+0.0231,r=0.9994.Conclusion The spectrophotometric method can be used in the determination of polysaccharides in Sangyuan Zhike capsule. |
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| Keywords: | Sangyuan Zhike capsule polysaccharides spectrophotometric method |
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