| HPLC法测定治伤软膏中苦参碱的含量 |
| |
| 引用本文： | 陈勇,陈碧莲,陆静娴.HPLC法测定治伤软膏中苦参碱的含量[J].解放军药学学报,2012,28(4):347-349. |
| |
| 作者姓名： | 陈勇 陈碧莲 陆静娴 |
| |
| 作者单位： | 浙江省食品药品检验所,浙江杭州,310004 |
| |
| 摘 要： | 目的建立HPLC法测定治伤软膏中苦参碱的含量测定方法。方法采用Kromasil KR100-5 C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-磷酸三乙胺溶液(取0.1%磷酸溶液,用三乙胺调节pH值至7.6)(22∶78)为流动相,流速为1 ml·min-1,检测波长为210 nm。结果苦参碱的线性范围为0.1248～2.496μg,回归方程为Y=1905.1 X-239 03,r=0.9999;平均回收率为98.79%,RSD为1.6%(n=9)。结论该方法结果准确,灵敏度高,重复性好,可用于治伤软膏的质量控制。
|
| 关 键 词： | 治伤软膏 苦参碱 HPLC |
HPLC Determination of Matrine in Zhishang Ointment |
| |
| CHEN Yong
,
CHEN Bi-lian
,
LU Jing-xian.HPLC Determination of Matrine in Zhishang Ointment[J].Pharmaceutical Journal of Chinese People's Liberation Army,2012,28(4):347-349. |
| |
| Authors: | CHEN Yong CHEN Bi-lian LU Jing-xian |
| |
| Institution: | Zhejiang Provincial Institute for Food and Drug Control,Hangzhou 310004,China |
| |
| Abstract: | Objective To establish a method for determination of matrine in Zhishang Ointment by HPLC.Methods Kromasil KR100-5 C18(250 mm×4.6 mm,5 μm)was used.The mobile phase consisted of acetonitrile and phosphoric acid-triethylamine(0.1% phosphoric acid adjusted to pH 7.6 with triethylamine)(22∶ 78).The flow rate was 1 ml · min-1.The detection wavelength was 210 nm.Results A good linear correlation of matrine was observed within the range of 0.1248-2.495 μg(r=0.9999).The average recovery was 98.79% with an RSD of 1.6%(n=9).Conclution This method is accurate,sensitive and repeatable,so it can be used for quality control of Zhishang ointment. |
| |
| Keywords: | Zhishang ointment matrine HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
