银杏叶提取物对大鼠肾缺血再灌注损伤的保护作用

目的 :研究银杏叶提取物对大鼠肾缺血再灌注损伤的保护作用。方法 :肾缺血40min再灌注3h建立大鼠肾缺血再灌注损伤模型 ,用不同剂量的银杏叶提取物预处理。通过检测肾皮质丙二醛含量、超氧化物歧化酶活性及血浆肌酐、尿素氮水平变化和电镜观察肾组织病理改变。结果 :缺血再灌注后 ,肾组织出现明显病理改变 ,肾皮质丙二醛含量升高 ,超氧化物歧化酶活性下降 ,血浆肌酐、尿素氮水平升高。银杏叶提取物预处理能降低血浆中肌酐、尿素氮水平以及肾皮质丙二醛含量 ,且肾皮质超氧化物歧化酶活性明显升高 ,与缺血再灌注组比较有显著性差异 (P<0 05)。电镜观察肾组织的病理改变明显好转。结论 :银杏叶提取物对肾缺血再灌注损伤有保护作用 ,其机制可能与清除自由基、减轻脂质过氧化有关。

Protective Effect of Ginkgo Biloba Extract on Renal Functions Injured by Ischemia- Reperfusion

OBJECTIVE:To study the protctive effect of ginkgo biloba extractEGBon the kindey in the case of isˉchemia-reperfusion injury.METHODS:The model of renal ischemia-reperfusion injury in male rats was made by ligation of left renal artery for40min and3h of reperfusion.The rats with pretreatment were fed with EGB at different doses prior to operation.The content of malonadialdihydeMDA,the activity of superoxide dismutaseSODin renal cortex and the levels of blood urea nitrogenBUN,creatinineCrin plasma were measured.The pathological changes of renal tissues were observed by electron microscope.RESULTS:After ischemia-reperfusion,the activity of SOD in renal cortex was decreased,however,the content of MDA in renal cortex and the levels of BUN,Cr in plasma were increased.Pathological changes induced by ischemia-reperfusion in renal tissues were observed clearly.The pretreatment of rats with EGB significantly prevented reduction of SOD activity and increase of MDA content in renal cortex,decreased elevation of concentration of BUN and Cr in plasˉma.Pathological changes of proximal tubular cells in rat kidneys induced by ischemia-reperfusion were also prevented by the pretreatment with EGB.CONCLUSION:EGB can protect rats from renal injuries caused by ischemia-reperfusion.The mechanism of protective effects of EGB may be related to preserving the activity of SOD and alleviating lipid peroxidation.