半环槽外固定器建立山羊胫骨缺损模型

目的 探索半环槽外固定器建立山羊胫骨段缺损模型.方法 山羊9只分3组,制备胫骨中下段20%的骨膜和骨缺损,半环槽外固定器外固定,术后第5、10、15周分别处死1组动物.用放射学、组织学方法评价骨缺损修复情况.结果 术后山羊胫骨段缺损处固定稳定,5、10、15周骨缺损处未见骨修复,15周时见骨断端硬化,放射学和组织学检查未见骨形成.结论 应用半环槽外固定器建立组织工程用大段骨缺损动物模型效果较好,山羊胫骨20%的骨膜和骨缺损不能自行修复.

Construction of goat tibial diaphyseal defect model by half-ring sulcated external fixator

Objective To construct goat tibial diaphyseal defect model by using half-ring sulcated external fixator.Methods Nine goats were divided into three groups.The 20 percent single tibial diaphyseal defect of every goat was made and fixed with half-ring sulcated external fixator.Animals in each group were killed respectively at 5 th,10 th,15 th week postoperatively.Bone formation was evaluated by X-ray and histology.Results The fixity was stable in goat tibial diaphyseal defect.No bone repair was found at the 5 th,10 th,15 th week postoperatively and sclerization at the broken ends of the fractured bone was observed at the 15 th week postoperatively but no bone formation was found.Conclusion Half-ring sulcated external fixator is a fair apparatus in constructing long bone defect model for tissue engineering repairing study and 20 percent goat tibial diaphyseal defect cannot be repaired by itself.