儿童脉管性疾病患者介入手术后外周血淋巴细胞染色体畸变观察

目的:评估儿童脉管性疾病患者在介入手术过程中接受的电离辐射(IR)对患儿染色体畸变的影响。方法:在知情同意的条件下，于介入术前和术后立刻采集26名患儿的外周血1.2 ml，培养制备染色体，分析外周血淋巴细胞染色体畸变情况。结果:儿童介入术后双着丝粒染色体+着丝粒环(dic+r)率明显增加，与术前比较，差异有统计学意义(...

Observation on chromosome aberration of peripheral blood lymphocytes in children with vascular diseases after interventional procedure

Objective To evaluate the effect of ionizing radiation on chromosome aberration of peripheral blood lymphocytes in children with vascular interventional procedure. Methods The chromosome aberration of peripheral blood lymphocytes was analyzed before and after vascular interventional procedure to assess the effect of low-dose ionizing radiation on chromosomes. Peripheral blood (PB) was collected using heparin as an anticoagulant from 26 patients before and after (within 10 minutes) vascular interventional surgery. Informed consent was obtained from the patients'' parents. Results Dicentric + centric ring (dic+r) formation increased significantly (U=647, P<0.01) in peripheral blood lymphocytes of children after interventional operation. No correlation was observed between the rate of dic+r and the cumulative dose (CD), dose area product (DAP), age, body weight, gender and the estimated effective dose E (P>0.05). There was no significant difference in dic+r rate of children between different genders, ages and lesion sites (P>0.05). The dic+r rate after interventional operation exceeded 40×10^-3 in 4 of 26 cases with the highest of 95.83×10^-3. Conclusions The ionizing radiation during children''s vascular interventional procedure might lead to the increase of chromosome aberration of peripheral blood lymphocytes and some children are abnormally sensitive. Great attention should be paid to the justification and radiation protection during interventional procedure for children.