桥接整合因子1在上皮性卵巢癌组织和细胞中的表达及其临床意义

目的:探讨桥接整合因子1 (BIN1)在上皮性卵巢癌(EOC)组织中的表达及其临床意义,以及BIN1对EOC细胞A2780增殖、迁移和侵袭的影响.方法:收集2017年7月至2018年1月河北医科大学第四医院手术切除的67例EOC患者的肿瘤组织及同期因其他妇科疾病手术切除的30例非肿瘤患者的卵巢组织(正常对照组)标本.用...

Expression and clinical significance of bridging intergrator 1 in epithelial ovarian cancer tissues and cells

Objective: To investigate the expression and clinical significance of bridging intergrator 1(BIN1) in epithelial ovarian cancer(EOC) tissues, and to explore its effect on the proliferation, migration and invasion of ovarian cancer A2780 cells. Methods: The tumor tissues of 67 patients with EOC who underwent tumor resection in the Fourth Hospital of Hebei Medical University from July2017 to January 2018 were collected. Ovarian tissues resected from 30 non-tumor patients with other gynecological diseases during the same period were collected as normal controls. Immunohistochemical staining was used to detect the expression of BIN1 in EOC tissues and non-tumor ovarian tissues. χ;test was used to analyze the correlation between the expression of BIN1 and various clinicopathological factors of EOC patients. Kaplan-Meier method was used to analyze the correlation between the expression of BIN1 and disease-free survival(DFS) or overall survival(OS) of patients. The mRNA and protein expression levels of BIN1 in EOC cells(SKOV3 and A2780) and human ovarian epithelial IOSE80 cells were detected by qPCR and WB. The BIN1 plasmid CMV-MCS-GFPSV40-neomycin-BIN1 and the empty plasmid CMV-MCS-GFP-SV40-Neomycin were respectively transfected into A2780 cells to construct BIN1 overexpressed cells and its control. The mRNA and protein expression levels of BIN1 in transfected cells were detected by q PCR and WB, respectively. The effects of BIN1 overexpression on the proliferation, migration and invasion of A2780 cells were detected by CCK-8 test, wound-healing assay and Transwell test, respectively. Results: The positive expression rate of BIN1 in EOC tissues was significantly lower than that in normal ovarian tissues(P<0.01). Low BIN1 expression was positively related with advanced postoperative pathological stage, worse histological grade, lymph node metastasis and peritoneal metastasis in patients with EOC(all P<0.05). The DFS and OS of the patients in the low BIN1 expression group were shorter than those in the high BIN1 expression group(all P<0.05). The m RNA and protein expression levels of BIN1 in SKOV3 and A2780 cells were significantly lower than those in IOSE80 cells(all P<0.01). Overexpression of BIN1 significantly inhibited the proliferation, migration and invasion of A2780 cells(P<0.05 or P<0.01). Conclusion: BIN1 is lowly expressed in EOC tissues and cells, and its low expression is related to the poor prognosis of EOC patients. Overexpression of BIN1 can inhibit the proliferation, migration and invasion ability of ovarian cancer A2780 cells.