| 应用生物素-链霉亲合素放大ELISA系统检测抗环瓜氨酸肽抗体 |
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| 引用本文： | 房国祥,吴云娟,王书侠,虞伟,李晓军. 应用生物素-链霉亲合素放大ELISA系统检测抗环瓜氨酸肽抗体[J]. 临床检验杂志, 2009, 27(1): 20-23 |
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| 作者姓名： | 房国祥 吴云娟 王书侠 虞伟 李晓军 |
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| 作者单位： | 江苏大学基础医学与医学技术学院,江苏镇江,212001;南京军区南京总医院解放军临床检验医学研究所,南京,210002;南京军区南京总医院解放军临床检验医学研究所,南京,210002 |
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| 基金项目： | 中国博士后科学基金资助项目(20060400940);;江苏省博士后科学基金资助项目(0602008C);;南京军区南京总医院重点课题(Z2008056) |
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| 摘 要： | 目的用生物素-链霉亲合素放大ELISA系统建立一种敏感、特异、经济简便的抗环瓜氨酸肽(CCP)抗体的测定法。方法用链霉亲合素(SA)预包被,使生物素化的CCP(bio-CCP)固相化,摸索检测抗CCP抗体的最优实验条件,并进行方法学考核和临床应用评价。结果本法对类风湿关节炎(RA)的诊断敏感性为69.4%,特异性为96.8%,阳性预测值为89.1%,阴性预测值为94.1%,阳性似然比为19.28,阴性似然比为0.317,Youden指数为0.658。与欧蒙试剂比对,两法所测抗CCP抗体吸光度/cutoff比值显著相关(n=61,r=0.9253,P<0.001)。在所统计的168例血清样本中,两法同时阳性者60例,同时阴性者105例,一致性百分率达98.2%,结果具有高度一致性(Kappa=0.961,P<0.001)。ROC曲线分析结果显示本法所测抗CCP抗体对RA诊断准确性达到了一个较高水平(AUCROC值=0.901)。在13例诊断为早期RA者中,有10例抗CCP抗体阳性(阳性率76.9%),未发现抗CCP抗体与RA患者临床症状、疾病活动指标(ESR)及RF水平有关。结论成功将生物素-链霉亲合素ELIS...
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| 关 键 词： | 环瓜氨酸肽 类风湿关节炎 酶联免疫吸附试验 生物素-链霉亲合素 |
Determination of anti-CCP antibody by biotin-streptavidin amplified ELISA system |
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| FANG Guo-xiang,,WU Yun-juan,WANG Shu-xia,YU Wei,LI Xiao-jun. Determination of anti-CCP antibody by biotin-streptavidin amplified ELISA system[J]. Chinese Journal of Clinical Laboratory Science, 2009, 27(1): 20-23 |
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| Authors: | FANG Guo-xiang WU Yun-juan WANG Shu-xia YU Wei LI Xiao-jun |
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| Affiliation: | 1.College of Basic Medicine & Medical Technology;Jiangsu University;Jiangsu;Zhenjiang212001;2.PLA Institute of Clinical Laboratory Medicine;Nanjing General Hospital of Nanjing Military Command;PLA;Nanjing210002;China |
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| Abstract: | Objective To develop a highly sensitive,specific and simple enzyme linked immunosorbent assay(ELISA)for determination of anti-cyclic citrullinated peptide(CCP)antibody.Methods By using the pattern of streptavidin pre-immobilization and conjugation with biotin-CCP(SA/Bio-CCP),the experimental conditions of ELISA for anti-CCP antibody were optimized and its methodology and results of clinical application were evaluated.Results The diagnostic sensitivity,specificity,positive/negative predictive value,positive/... |
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| Keywords: | cyclic citrullinated peptide rheumatoid arthritis enzyme linked immunosorbent assay biotin-straptavidin |
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