X射线照射诱导鼻咽癌细胞hMSH2的表达

目的研究X射线照射对鼻咽癌CNE-1细胞错配修复基因hMSH2表达的影响,探讨放射损伤后肿瘤细胞的DNA修复机制。方法应用逆转录-PCR(RT-PCR)、免疫细胞化学及蛋白免疫印迹(Western blot)方法,检测X射线照射后对照组和实验组(照射总剂量分别为0Gy和10Gy)细胞中hM-SH2基因mRNA及蛋白表达。结果实验组细胞hMSH2基因mRNA及蛋白表达在照射终止后逐渐上调,其表达较对照组显著增强(P<0.01)。结论X射线照射可诱导鼻咽癌细胞hMSH2的表达,有助于放射损伤后肿瘤细胞DNA修复,这可能是肿瘤放疗敏感性降低的原因之一。

X-irradiation Induces hMSH2 Expression in Nasopharyngeal Carcinoma Cells

Objective To study the effect of X-irradiation on hMSH2 expression in nasopharyngeal carcinoma(NPC) CNE-1 cells, and explore mechanisms of DNA mismatch repair after irradiation. Methods Control and experimental cells were irradiated with 0Gy and 10Gy respectively,and hMSH2 expression was tested by RT-PCR, immunocytochemistry and Western blot. Results The expression of hMSH2 mRNA and protein in experimental cells were increased after irradiation, and were significantly higher than those in control cells(P< 0.01 ).Conclusion X-irradiation may induce hMSH2 expression in NPC cells, which contributes to DNA repair and maybe responsible for reduction of radio-sensitivity after irradiation.