复方雪荔颗粒及其有效部位的利尿及体外抑菌作用的实验研究

目的观察复方雪荔颗粒及其有效部位的利尿、抑菌作用。方法大鼠灌胃给予复方雪荔颗粒原液(40,20,10g.kg-1)及各萃取部位,用大鼠代谢笼法分析大鼠尿中Na+、K+、Cl-离子的含量;用浓度梯度稀释法测定其对不同菌株的最低抑菌浓度(MIC)。结果复方雪荔颗粒原液(40,20g.kg-1)剂量组及乙酸乙酯层的(40,20g.kg-1)剂量组,均能显著增加水负荷大鼠5h的尿量,且尿中Na+、K+、Cl-离子的含量显著增加;原液对大肠杆菌、金葡菌的MIC分别为0.25,0.50g.mL-1;雪荔颗粒的乙酸乙酯层有效部位对大肠杆菌、金葡菌的MIC分别为0.5,1.0g.mL-1。结论复方雪荔颗粒原液及其有效部分均有显著的利尿及抑菌作用。

Experimental study on the diuretic effect and bacterial activity in vitro of compound Salvia plebeia granules and its different effective parts in rats

Objective To study on the diuretic effects and bacterial activity in vitro of compound Salvia plebeian granules and its different effective parts in rats.Methods The diuretic effects of the compound Salvia plebeian granules(CSPG)and every extracted part were studied by using rat metabolic cage and analyzing the contents of Na~+、K~+、Cl~- in rat's urine.MICs of CSPG and every extracted part on different bacteria strains were determined by the method of concentration gradient dilution.Results In diuretic experiment,doses of 40,20 g·kg~(-1) of both CSPG and extraction of acetic ether(AE,effective part of CSPG)can conspicuously increase the urine volume of water load rats and also increase the contents of Na~+、K~+、Cl~- in rats'urine.The MICs of CSPG on E.coli and Staphylococcus aureus were 0.25 g·mL~(-1) and 0.50 g·mL~(-1).The MICs of AE on E.coli and Staphylococcus aureus were 0.5 g·mL~(-1) and 1.0 g·mL~(-1).Conclusion CSPG and its effective parts have the diuretic effects in vitro and bacterial activity.